Development of skeletal muscle fibrosis in a rodent model of cancer cachexia.
Development of skeletal muscle fibrosis in a rodent model of cancer cachexia.
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DOI：
10.1002/cbf.3797
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发表时间：
2023-06
影响因子：
3.6
通讯作者：
Greene, Nicholas P.
中科院分区：
文献类型：
--
作者：
Washington, Tyrone A.;Schrems, Eleanor R.;Haynie, Wesley S.;Rosa-Caldwell, Megan E.;Brown, Jacob L.;Saling, Landen;Lim, Seongkyun;Perry Jr, Richard A.;Brown, Lemuel A.;Lee, David E.;Greene, Nicholas P.
恶病质的特点是瘦体重的损失，其进展导致癌症患者生活质量恶化和预后恶化。然而，纤维化在早期阶段和恶病质发展中的作用和影响尚不清楚。本研究的目的是确定在恶病质形成过程中是否发生纤维化，并在两性中进行评估。雌性和雄性C57BL6/J小鼠在8周龄时注射磷酸盐缓冲盐水或Lewis肺癌（LLC），肿瘤发展1、2、3或4周。3周和4周的雌性荷瘤小鼠肿瘤生长分为两组，分别分为高瘤组和低瘤组。体外分析C2C12和3T3细胞暴露于LLC条件培养基。采用免疫组织化学和定量聚合酶链反应（qPCR）分析骨骼肌纤维化和纤维化相关信号。雌性小鼠1周、LT和HT组骨骼肌胶原沉积增加。然而，在雄性小鼠中，胶原沉积仅在第4周组增加。一般来说，雌性小鼠在注射llc后1周开始出现细胞外基质（ECM）相关基因的早期改变。而这在男性中没有发现。尽管在两性中，总体肿瘤负荷与恶病质的发展密切相关，但纤维化的发展并非如此。雄性小鼠没有表现出与雌性小鼠相反的早期ecm相关基因的改变。
Cachexia is characterized by losses in lean body mass and its progression results in worsened quality of life and exacerbated outcomes in cancer patients. However, the role and impact of fibrosis during the early stages and development of cachexia in under-investigated. The purpose of this study was to determine if fibrosis occurs during cachexia development, and to evaluate this in both sexes. Female and male C57BL6/J mice were injected with phosphate-buffered saline or Lewis Lung Carcinoma (LLC) at 8-week of age, and tumors were allowed to develop for 1, 2, 3, or 4 weeks. 3wk and 4wk female tumor-bearing mice displayed a dichotomy in tumor growth and were reassigned to high tumor (HT) and low tumor (LT) groups. In vitro analyses were also performed on cocultured C2C12 and 3T3 cells exposed to LLC conditioned media. Immunohistochemistry and quantitative polymerase chain reaction (qPCR) analysis were used to investigate fibrosis and fibrosis-related signaling in skeletal muscle. Collagen deposition in skeletal muscle was increased in the 1wk, LT, and HT groups in female mice. However, collagen deposition was only increased in the 4wk group in male mice. In general, female mice displayed earlier alterations in extracellular matrix (ECM)-related genes beginning at 1wk post-LLC injection. Whereas this was not seen in males. While overall tumor burden is tightly correlated to cachexia development in both sexes, fibrotic development is not. Male mice did not exhibit early-stage alterations in ECM-related genes contrary to what was noted in female mice.
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DOI：
10.1002/jcsm.12354
发表时间：
2018-10
期刊：
Journal of cachexia, sarcopenia and muscle
影响因子：
--
作者：
Brown JL;Lee DE;Rosa-Caldwell ME;Brown LA;Perry RA;Haynie WS;Huseman K;Sataranatarajan K;Van Remmen H;Washington TA;Wiggs MP;Greene NP
通讯作者：
Greene NP
DOI：
10.1002/jcsm.12232
发表时间：
2017-12
期刊：
Journal of cachexia, sarcopenia and muscle
影响因子：
--
作者：
Brown JL;Rosa-Caldwell ME;Lee DE;Blackwell TA;Brown LA;Perry RA;Haynie WS;Hardee JP;Carson JA;Wiggs MP;Washington TA;Greene NP
通讯作者：
Greene NP
DOI：
10.1152/ajpheart.00106.2015
发表时间：
2015-08-15
影响因子：
4.8
作者：
Devine, Raymond D.;Bicer, Sabahattin;Wold, Loren E.
通讯作者：
Wold, Loren E.
影响因子：
4.1
作者：
LIGHT, N;CHAMPION, AE
通讯作者：
CHAMPION, AE
影响因子：
5.6
作者：
Caja L;Dituri F;Mancarella S;Caballero-Diaz D;Moustakas A;Giannelli G;Fabregat I
通讯作者：
Fabregat I