Aktivitas Antifungi Ekstrak Etanol Daun Salam (Syzygium polyanthum (Wight) Walp.) terhadap Pertumbuhan Jamur Candida albicans secara in vitro
Aktivitas Antifungi Ekstrak Etanol Daun Salam (Syzygium polyanthum (Wight) Walp.) terhadap Pertumbuhan Jamur Candida albicans secara in vitro
复制标题
Aktivitas Antifungi Ekstrak Etanol Daun Salam (Syzygium polyanthum (Wight) Walp.) terhadap Pertumbuhan Jamur Candida albicans secara 体外
DOI：
10.20884/1.mib.2012.29.2.238
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发表时间：
2012
影响因子：
4.9
通讯作者：
Ria Engriyani Ria Engriyani
中科院分区：
文献类型：
--
作者：
A. Fitriani;Y. Hamdiyati;Ria Engriyani Ria Engriyani
研究了月桂叶乙醇提取物对白色念珠菌的体外抑菌活性。多花参叶对皮肤真菌病原具有潜在的抗真菌作用。本研究旨在研究多花参叶乙醇提取物对白色念珠菌生长的抑制作用。采用圆盘扩散法和宏观稀释法进行活性试验。本研究采用0.5%、1%、1.5%、2%和2.5% （w/v）乙醇浓度的多花参叶提取物。阴性对照用1% DMSO，阳性对照用酮康唑30 mg/mL。结果表明，蓼叶乙醇提取物具有一定的抗真菌活性。基于气相色谱分析结果对多花参叶乙醇提取物中含有萜类和脂肪酸等化学成分进行分析。多花参叶乙醇提取物在1%浓度（w/v）下的抑菌带直径为9.32±0.21 mm。在0.5% （w/v）浓度下多花参叶乙醇提取物的最小抑菌浓度（MIC）值和1% （w/v）浓度下的最小杀菌浓度（MFC）值。
Antifungal activity of the ethanol extract of bay-leaf ( Syzygium polyanthum (Wight) Walp.) against growth of the fungus Candida albicans in vitro has been conducted. Leaves of S. polyanthum has been known to have potential as antifungal for skin fungal pathogen. This study aims to determine antifungal activity of the ethanol extract of S. polyanthum leaves against growth of C. albicans . Activity test was done by disc-diffusion method and macro-dilution. This study used concentrations of ethanol leaf extract of S. polyanthum of 0.5%, 1%, 1.5%, 2%, and 2.5% (w/v). Negative control using 1% DMSO and positive controls using ketoconazole 30 mg/mL. The results shows that ethanol extract of leaves of S. polyanthum have activity as an antifungal. Ethanol extract of leaves of S. polyanthum based on the results of GCMS analysis of compounds containing chemical compounds such as terpenoids and fatty acids. Ethanol extract of leaves of S. polyanthum showed the highest inhibition zone diameter at a concentration of 1% (w/v) of 9.32 ± 0.21 mm. Value of Minimum inhibitory Concentration (MIC) for ethanol leaf extract of S. polyanthum present in a concentration of 0.5% (w/v) and the value of Minimum Fungicidal Concentration (MFC) present in a concentration of 1% (w/v).