Selective Labeling Aldehydes in DNA
Selective Labeling Aldehydes in DNA
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选择性标记 DNA 中的醛
DOI：
10.1021/acs.analchem.8b04822
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发表时间：
2018
影响因子：
7.4
通讯作者：
Zhou Xiang
中科院分区：
文献类型：
--
作者：
Liu Chaoxing;Luo Xiaomeng;Chen Yuqi;Wu Fan;Yang Wei;Wang Yafen;Zhang Xiong;Zou Guangrong;Zhou Xiang
设计并合成了萘酰亚胺羟胺探针，用于选择性标记 DNA 中存在的全部天然醛，包括 5-甲酰胞嘧啶、5-甲酰尿嘧啶和脱碱基位点。详细研究了生成的核苷的荧光特性，并与他人讨论了羟胺、胺基对DNA中醛的反应活性，这对于设计用于选择性标记DNA的化学品具有重要参考意义。
A naphthalimide hydroxylamine probe has been designed and synthesized to selectively label the whole natural aldehydes present in DNAs including 5-formylcytosine, 5-formyluracil, and abasic sites. The fluorescence characteristics of the generated nucleosides have been examined in detail, and the reaction activities of hydroxylamine, amine groups toward aldehydes in DNA have been discussed with others, which will be a vital reference for designing chemicals for selective labeling of DNAs.