gamma-Aminobutyric acid-activated chloride channels in rats selectively bred for differential acute sensitivity to alcohol.
gamma-Aminobutyric acid-activated chloride channels in rats selectively bred for differential acute sensitivity to alcohol.
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选择性饲养的大鼠中的γ-氨基丁酸激活的氯离子通道对酒精有不同的急性敏感性。
DOI：
10.1111/j.1530-0277.1991.tb01858.x
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Draski,LJ
中科院分区：
文献类型：
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作者：
Allan,AM;Mayes,GG;Draski,LJ
研究人员在全脑膜囊泡（微囊）中评估了各种镇静催眠药对 GABA 介导的氯离子通量的影响，这些囊泡由选择性饲养的对急性催眠剂量酒精的高敏感性（HAS）和低敏感性（LAS）的大鼠制备。与 LAS 大鼠相比，HAS 大鼠对戊巴比妥、苯巴比妥、氟硝西泮和乙醇对 GABA 介导的氯离子通量的影响更敏感。没有观察到 GABA 刺激的氯通量的线之间存在差异。还测量了 1-[3H]-苯基-4-丁基-2,6,7-三氧杂双环(2.2.2)辛烷 ([3H]-TBOB) 和 [3H]-地西泮结合的调节。这些品系在戊巴比妥、苯巴比妥、蝇蕈醇或印防己毒素对[3H]-TBOB结合的抑制方面没有差异。尽管这些细胞系显示出几乎相同的 [3H]-地西泮结合的 KO 和 Bmax，但在 HAS 大鼠制备的膜中，GABA 激动剂蝇蕈醇是比 LAS 大鼠制备的膜更有效的 [3H]-地西泮结合刺激剂。这些发现是根据之前使用其他选定品系的工作进行讨论的。
Effects of various sedative hypnotic agents on GABA‐mediated chloride flux were evaluated in whole brain membrane vesicles (microsacs) prepared from rats selectively bred for high (HAS) and low sensitivity (LAS) to an acute hypnotic dose of alcohol. The HAS rats were more sensitive to the effects of pentobarbital, phenobarbital, flunitrazepam, and ethanol on GABA‐mediated chloride flux compared with the LAS rats. No differences between the lines in GABA‐stimulated chloride flux were observed. Modulation of 1‐[3H]‐phenyl‐4‐butyl‐2,6,7‐trioxabicyclo(2.2.2)octane([3H]‐TBOB) and [3H]‐diazepam binding also was measured. The lines did not differ in inhibition of [3H]‐TBOB binding by pentobarbital, phenobarbital, muscimol or picrotoxin. Although the lines displayed almost identicalKOand Bmaxfor [3H]‐diazepam binding, the GABA agonist, muscimol, was a more potent stimulator of [3H]‐diazepam binding in membranes prepared from HAS rats than from LAS rats. These findings are discussed in light of previous work using other selected lines.