Single-Molecule Approach to DNA Minor-Groove Association Dynamics
Single-Molecule Approach to DNA Minor-Groove Association Dynamics
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DOI：
10.1002/anie.201201099
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发表时间：
2012-01-01
影响因子：
16.6
通讯作者：
Novo, Mercedes
中科院分区：
文献类型：
--
作者：
Bordello, Jorge;Sanchez, Mateo I.;Novo, Mercedes
进入沟槽：荧光相关光谱显示，双联苯脒小沟槽结合剂BBA‐OG（蓝带绿星，见图）与dsDNA的结合动力学过程不是由扩散控制的，而是由结合剂在特定位点（红色）插入沟槽所控制的，如结合事件每一步的速率常数所示
Getting in the groove: Fluorescence correlation spectroscopy reveals that the dynamics of the association process of the bisbenzamidine minor‐groove binder BBA‐OG (blue with green star, see scheme) to dsDNA is not controlled by diffusion, but by the insertion of the binder into the groove at the specific site (red), as shown by the rate constants for each step of the binding event