Metabolite profile of lactic acid-fermented soymilk.
Metabolite profile of lactic acid-fermented soymilk.
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DOI：
10.4236/fns.2018.911095
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发表时间：
2018-01-01
期刊：
影响因子：
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通讯作者：
Fukuda, M.
中科院分区：
文献类型：
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作者：
Kobayashi, M.;Shima, T.;Fukuda, M.
由德氏乳杆菌亚种发酵而成的富含异黄酮苷元的豆奶。德氏乳杆菌 TUA-4408 L (F4408) 菌株比德氏乳杆菌发酵的低异黄酮苷元豆奶具有更强的脂质代谢调节作用。 delbrueckii，菌株 TUA-4404 L (F4404)。因此，异黄酮苷元以外的发酵产物也可能发挥脂质代谢调节作用。因此，本研究旨在通过代谢组学方法比较豆奶（SM）、F4404和F4408中的代谢物。与 SM 相比，F4404 和 F4408 中的各种苷元（包括异黄酮、黄酮、黄酮醇和黄烷酮）均有所增加。 F4408 中异黄酮和黄烷酮苷元的增加比 F4404 中更大。两种发酵豆奶中某些类型的二肽和游离氨基酸，尤其是鸟氨酸都有所增加。 F4408 中游离氨基酸尤其是鸟氨酸的增加高于 F4404。因此，F4408表现出比F4404更强的糖苷酶和蛋白酶活性。众所周知，乳酸发酵产生的功能成分可以改善脂质代谢。因此，表明在发酵豆奶中，尤其是使用TUA-4408 L发酵的豆奶中，不仅异黄酮苷元，而且其他功能成分也发挥脂质代谢调节作用。
Isoflavone aglycone-rich soymilk fermented by Lactobacillus delbrueckii subsp. delbrueckii, strain TUA-4408 L (F4408) has stronger lipid metabolism-modulating effects than isoflavone aglycone-poor soymilk fermented by Lactobacillus delbrueckii subsp. delbrueckii, strain TUA-4404 L (F4404). As such, fermentation products other than isoflavone aglycones may also exert lipid metabolism-modulating effects. Therefore, the present study aimed to compare the metabolites in soymilk (SM), F4404, and F4408 via a metabolomics approach. Various aglycones including isoflavones, flavones, flavonols, and flavanones increased in both F4404 and F4408 compared with SM. The increases in isoflavone and flavanone aglycones were greater in F4408 than in F4404. Some types of dipeptides and free amino acids, especially ornithine, increased in both fermented soymilks. The increase in free amino acids, especially ornithine, was higher in F4408 than in F4404. Thus, F4408 exhibited stronger glycosidase and protease activities than F4404. Functional components produced by lactic acid fermentation are known to improve lipid metabolism. Therefore, it is suggested that not only isoflavone aglycones but also other functional components exert lipid metabolism-modulating effects in fermented soymilks, especially soymilk fermented using TUA-4408 L.