A serum amyloid P-binding hydrogel speeds healing of partial thickness wounds in pigs.
A serum amyloid P-binding hydrogel speeds healing of partial thickness wounds in pigs.
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DOI：
10.1111/j.1524-475x.2009.00482.x
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发表时间：
2009-05
期刊：
影响因子：
--
通讯作者：
Davis SC
中科院分区：
文献类型：
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作者：
Gomer RH;Pilling D;Kauvar LM;Ellsworth S;Ronkainen SD;Roife D;Davis SC
在伤口愈合过程中，一些循环单核细胞进入伤口，分化成成纤维细胞样细胞（称为纤维细胞），然后似乎进一步分化成肌成纤维细胞，这些细胞在胶原蛋白沉积、细胞因子释放和伤口收缩中起关键作用。单核细胞向纤维细胞的分化受到血清淀粉样蛋白P（SAP）的抑制。因此，在伤口部位消耗SAP可以加速伤口愈合。SAP在Ca 2+存在下与某些类型的琼脂糖结合。我们发现人SAP与琼脂糖结合的KD为7×10− 8 M，Bmax为2.1 μg SAP/mg湿重琼脂糖。混合琼脂糖1：5 w/v与30 μg/mL人SAP（正常血清中的平均SAP浓度）在含有2 mM Ca 2+的缓冲液中的混合物将游离SAP浓度降低至约0.02 μg/mL，远低于抑制纤维细胞分化的浓度。与水凝胶敷料和泡沫敷料相比，含有这种琼脂糖和Ca 2+的敷料显著增加了猪部分厚度伤口的伤口愈合速度。这表明琼脂糖/Ca 2+敷料可能有利于人类伤口愈合。
During wound healing, some circulating monocytes enter the wound, differentiate into fibroblast-like cells called fibrocytes, and appear to then further differentiate into myofibroblasts, cells that play a key role in collagen deposition, cytokine release, and wound contraction. The differentiation of monocytes into fibrocytes is inhibited by the serum protein serum amyloid P (SAP). Depleting SAP at a wound site thus might speed wound healing. SAP binds to some types of agarose in the presence of Ca2+. We found that human SAP binds to an agarose with a KD of 7×10−8M and a Bmax of 2.1 μg SAP/mg wet weight agarose. Mixing this agarose 1: 5 w/v with 30 μg/mL human SAP (the average SAP concentration in normal serum) in a buffer containing 2mM Ca2+ reduced the free SAP concentration to ~0.02 μg/mL, well below the concentration that inhibits fibrocyte differentiation. Compared with a hydrogel dressing and a foam dressing, dressings containing this agarose and Ca2+ significantly increased the speed of wound healing in partial thickness wounds in pigs. This suggests that agarose/Ca2+ dressings may be beneficial for wound healing in humans.