Effect of interleukin-2 on synthesis of B cell activating factor belonging to the tumor necrosis factor family (BAFF) in human peripheral blood mononuclear cells
Effect of interleukin-2 on synthesis of B cell activating factor belonging to the tumor necrosis factor family (BAFF) in human peripheral blood mononuclear cells
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DOI：
10.1016/j.cyto.2008.06.005
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发表时间：
2008-10-01
期刊：
影响因子：
3.8
通讯作者：
Takeuchi, Tsutomu
中科院分区：
文献类型：
--
作者：
Suzuki, Katsuya;Setoyama, Yumiko;Takeuchi, Tsutomu
B细胞激活因子属于肿瘤坏死因子家族(BAFF)，是B细胞生存、成熟和激活所必需的细胞因子。BAFF的过度表达导致小鼠狼疮样疾病，而人类狼疮患者血清BAFF水平升高。然而，对BAFF的合成及其调控知之甚少。在这项研究中，我们研究了一系列炎性细胞因子对体外培养的人外周血单个核细胞(PBMC)产生BAFF的影响。我们发现白细胞介素2(IL-2)强烈且呈剂量依赖性地刺激PBMCs合成BAFF，而抗IL-2抗体可中和这一作用。此外，T细胞和NK细胞在IL-2刺激下产生BAFF。由此可见，IL-2是对人外周T细胞和NK细胞合成BAFF有积极作用的调节性细胞因子之一。在狼疮等病理状态下，IL-2的持续过量产生可能导致PBMC中BAFF合成的上调。(C)2008爱思唯尔有限公司。保留所有权利。
B cell activating factor belonging to the tumor necrosis factor family (BAFF) is a cytokine, indispensable for B cell survival, maturation, and activation. Over-expression of BAFF leads to lupus like disease in mice and the serum level of BAFF is elevated in human lupus. However, little is known about BAFF synthesis and its regulation. In this study, we examined the effects of a series of inflammatory cytokines on BAFF production in human peripheral blood mononuclear cells (PBMCs) in vitro. We found interleukin-2 (IL-2) strongly and dose-dependently stimulated BAFF synthesis in PBMCs, and an anti-IL-2 antibody neutralized the effect. Furthermore, T and NK cells produced BAFF with IL-2 stimulation. From these observations, IL-2 is one of the regulatory cytokines having a positive effect on BAFF synthesis in human peripheral T and NK cells. Persistent over-production of IL-2 might lead to up-regulation of BAFF synthesis in PBMCs in pathological conditions Such as lupus. (C) 2008 Elsevier Ltd. All rights reserved.