Occurrence of recombinant genotypes of Anisakis simplex s.s. and Anisakis pegreffii (Nematoda: Anisakidae) in an area of sympatry
Occurrence of recombinant genotypes of Anisakis simplex s.s. and Anisakis pegreffii (Nematoda: Anisakidae) in an area of sympatry
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DOI：
10.1016/s1567-1348(03)00073-x
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发表时间：
2003-09-01
影响因子：
3.2
通讯作者：
D'Amelio, S.
中科院分区：
文献类型：
--
作者：
Abollo, E.;Paggi, L.;D'Amelio, S.
从伊比利亚半岛沃茨采集的鱼类和搁浅鲸类沿着的异尖线虫种群在形态上被鉴定为对应于异尖线虫单纯复合体。为了实现分子鉴定和分析遗传变异的程度，整个ITS（ITS 1，5.8S rDNA基因和ITS 2）和线粒体小亚基rRNA的PCR扩增和测序。用HinfI和HhaI对扩增的ITS区进行双列杂交，可以鉴定出三种不同的基因型，分别属于A.单纯性s.s.，A. pegreffii和尚未描述的重组基因型。重组基因型的ITS序列显示在比对序列的240和256位存在杂合子C/T。另外，线粒体DNA序列分析表明重组基因型存在不同的亲本来源。为了检验它们是否是在A. pegreffii和A.辛普利斯普湾s.，和/或不完全协同进化的存在，也收集了三个样品作为对照，在隔离的地理区域，其中A.辛普利斯普湾S.和A.不发生Pegreffii。结果支持的假设，重组个体可能是种间杂交的产物，并描述伊比利亚半岛沃茨作为一个杂交区的两个兄弟物种。(C)2003年爱思唯尔科学B。V.保留所有权利。
The anisakid nematode populations collected from fish and stranded cetaceans along from Iberian Peninsula waters were morphologically identified as corresponding to the Anisakis simplex complex. In order to realise their molecular identification and to analyse the extent of genetic variation, the entire ITS (ITS1, 5.8S rDNA gene and ITS2) and the mitochondrial small subunit of rRNA were pcr-amplified and sequenced. Digestions of the amplified its region with HinfI and HhaI allowed the identification of three different genotypes, belonging to A. simplex s.s., A. pegreffii and a yet not described recombinant genotype. The ITS sequences of the recombinant genotypes showed the presence of heterozygotes C/T at position 240 and 256 of the aligned sequence. Otherwise, the analysis of mtDNA sequences showed the existence of a different parental origin for recombinant genotypes. In order to check if they can be the products of a polymorphism normally occurring both in A. pegreffii and in A. simplex s. s., and/or the existence of an incomplete concerted evolution, three samples were also collected as controls in isolated geographic areas, where sympatric coexistence between A. simplex s. s. and A. pegreffii does not occur. The results supports the hypothesis that the recombinant individuals may be a product of interspecific hybridisation, and describe the Iberian Peninsula waters as a hybrid zone for the two sibling species. (C) 2003 Elsevier Science B. V. All rights reserved.