Tensile force-induced cytoskeletal remodeling: Mechanics before chemistry
Tensile force-induced cytoskeletal remodeling: Mechanics before chemistry
复制标题
DOI：
10.1371/journal.pcbi.1007693
复制
发表时间：
2020-06-01
影响因子：
4.3
通讯作者：
Jiang, Yi
中科院分区：
文献类型：
--
作者：
Li, Xiaona;Ni, Qin;Jiang, Yi
了解细胞重塑响应机械刺激是一个关键步骤，阐明机械激活的生化信号通路。实验证据表明，外部压力诱导的亚细胞适应是通过动态的细胞骨架重组来完成的。为了研究参与机械信号转导的亚细胞结构之间的相互作用，我们结合实验数据和计算模拟来评估肌动蛋白细胞骨架网络的实时机械适应。肌动蛋白细胞骨架成像的同时，作为一个外部的张力施加到活的血管平滑肌细胞使用纤连蛋白功能化的原子力显微镜探针。此外，我们进行了计算模拟下的外部张力的活性细胞骨架网络。实验数据和模拟结果表明，在丝束形成过程中，机械结构适应发生在化学适应之前：肌动蛋白纤维首先通过初始化各向异性纤维取向而沿外力方向排列，然后网络的化学演化遵循各向异性结构，进一步发展出类似于斜纹的几何形状。我们的研究结果提出了一个替代的两步解释形成肌动蛋白束由于机械刺激，并提供了新的见解机械转导的机制。
Understanding cellular remodeling in response to mechanical stimuli is a critical step in elucidating mechanical activation of biochemical signaling pathways. Experimental evidence indicates that external stress-induced subcellular adaptation is accomplished through dynamic cytoskeletal reorganization. To study the interactions between subcellular structures involved in transducing mechanical signals, we combined experimental data and computational simulations to evaluate real-time mechanical adaptation of the actin cytoskeletal network. Actin cytoskeleton was imaged at the same time as an external tensile force was applied to live vascular smooth muscle cells using a fibronectin-functionalized atomic force microscope probe. Moreover, we performed computational simulations of active cytoskeletal networks under an external tensile force. The experimental data and simulation results suggest that mechanical structural adaptation occurs before chemical adaptation during filament bundle formation: actin filaments first align in the direction of the external force by initializing anisotropic filament orientations, then the chemical evolution of the network follows the anisotropic structures to further develop the bundle-like geometry. Our findings present an alternative two-step explanation for the formation of actin bundles due to mechanical stimulation and provide new insights into the mechanism of mechanotransduction.