Cloning and radiation hybrid mapping of bovine toll-like receptor-4 (TLR-4) signaling molecules
Cloning and radiation hybrid mapping of bovine toll-like receptor-4 (TLR-4) signaling molecules
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DOI：
10.1016/j.vetimm.2006.03.003
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发表时间：
2006-08-15
影响因子：
1.8
通讯作者：
Bannerman, Douglas D.
中科院分区：
文献类型：
--
作者：
Connor, Erin E.;Cates, Elizabeth A.;Bannerman, Douglas D.
Toll样受体(TLR)-4是内毒素的跨膜受体，内毒素是革兰氏阴性菌外膜的一种高度促炎成分。到目前为止，TLR-4信号通路的分子在牛身上还没有得到很好的表征。本研究的目的是克隆牛TLR-4信号转导相关基因CASP8、IRAK1、LY96(MD-2)、TICAM2、TIRAP、TOLLIP和TRAF 6的全长编码区，并利用辐射杂交定位技术对这些基因以及MyD88和TICAM1进行定位。这项工作的结果表明，与之前公布的LY96牛序列和GenBank数据库中TIRAP的预测序列不同，该序列基于牛基因组的最新组装。此外，基于BLAU_2.0基因组组装发布的实际和预测的染色体图位置之间的差异被发现，尽管图的位置与基于当前牛-人比较图的预测位置一致。牛的氨基酸序列与人和小鼠序列的比对显示出很大范围的保守性，从52%到93%。总体而言，这项工作将有助于牛基因组序列的组装和注释，鉴定与宿主先天性免疫有关的关键基因的变异，并促进牛TLR-4信号通路的研究。(C)2006爱思唯尔B.V.保留所有权利。
Toll-like receptor (TLR)-4 is a transmembrane receptor for lipopolysaccharide, a highly pro-inflammatory component of the outer membrane of Gram-negative bacteria. To date, molecules of the TLR-4 signaling pathway have not been well characterized in cattle. The goal of this study was to clone and sequence the full-length coding regions of bovine genes involved in TLR-4 signaling including CASP8, IRAK1, LY96 (MD-2), TICAM2, TIRAP, TOLLIP and TRAF 6 and to position these genes, as well as MyD88 and TICAM1, on the bovine genome using radiation hybrid mapping. Results of this work indicate differences with a previously published bovine sequence for LY96 and a predicted sequence in the GenBank database for TIRAP based on the most recent assembly of the bovine genome. In addition, discrepancies between actual and predicted chromosomal map positions based on the Blau_2.0 genome assembly release were identified, although map positions were consistent with predicted locations based on the current bovine-human comparative map. Alignment of the bovine amino acid sequences with human and murine sequences showed a broad range in conservation, from 52 to 93%. Overall, this work should assist in the assembly and annotation of the bovine genome sequence, the identification of variations in genes critically involved in host innate immunity, and facilitate the study of TLR-4 signaling pathways in cattle. (c) 2006 Elsevier B.V. All rights reserved.