NaCl flux between apical and basolateral side recruits claudin-1 to tight junction strands and regulates paracellular transport
NaCl flux between apical and basolateral side recruits claudin-1 to tight junction strands and regulates paracellular transport
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DOI：
10.1016/j.bbrc.2010.02.002
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发表时间：
2010-03-12
影响因子：
3.1
通讯作者：
Marunaka, Yoshinori
中科院分区：
文献类型：
--
作者：
Tokuda, Shinsaku;Miyazaki, Hiroaki;Marunaka, Yoshinori
在多细胞生物中，上皮细胞分离并分裂内部环境，维持每个隔室的适当条件。为了维持这些区室的内稳态，claudin，紧密连接中的主要细胞粘附分子（TJs），通过细胞旁通路（屏障功能）调节几种物质的运动。在这项研究中，我们研究了几种物质在根尖和基底外侧之间的流动对细胞旁转运和TJ蛋白定位的影响。从根尖到基底外侧的NaCl通量增加了细胞旁电导（Gp），并通过与occludin（一种集中在TJ链上的TJ蛋白）的共定位，将claudin-1从侧边细胞膜募集到根尖端。相反方向的蔗糖通量与NaCl通量抑制了这些反应，相同方向的蔗糖通量与NaCl增加了Gp的增加，而10-kDa葡聚糖抑制了这些反应，无论给药的方向如何。我们目前的研究结果表明，TJ蛋白的定位和屏障功能取决于根尖和基底外侧的环境差异。(C) 2010爱思唯尔公司版权所有。
In multicellular organisms, epithelia separate and divide the internal environment maintaining appropriate conditions in each compartment. To maintain homeostasis in these compartments, claudins, major cell adhesion molecules in tight junctions (TJs), regulate movements of several substances through the paracellular pathway (barrier function). In this study, we investigated effects of the flux of several substances between apical and basolateral side on paracellular transport and TJ protein localization. NaCl flux from apical to basolateral side increased paracellular conductance (Gp) and recruited claudin-1 from lateral cell membrane to the apical end with the colocalization with occludin, one of the TJ proteins concentrated at TJ strands. Oppositely-directed flux of sucrose against NaCl flux inhibited these reactions and same directional flux of sucrose with NaCl enhanced the increase of Gp, whereas 10-kDa dextran inhibited these reactions regardless of the side of administration. Our present findings indicated that TJ protein localization and barrier function are regulated depending on the environmental differences between apical and basolateral side. (C) 2010 Elsevier Inc. All rights reserved.