INDUCTION OF BETA-AMYLOID PRECURSOR PROTEIN ISOFORM MESSENGER-RNAS BY BFGF IN ASTROCYTES
INDUCTION OF BETA-AMYLOID PRECURSOR PROTEIN ISOFORM MESSENGER-RNAS BY BFGF IN ASTROCYTES
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DOI：
10.1097/00001756-199306000-00054
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发表时间：
1993-06-01
期刊：
影响因子：
1.7
通讯作者：
PATEL, AJ
中科院分区：
文献类型：
--
作者：
GRAY, CW;PATEL, AJ
β -淀粉样蛋白前体蛋白（APP）的三种主要转录本均在星形胶质细胞中表达。APP695、APP751和APP770异构体mrna的比例为1:4:2。经bFGF和EGF处理后，培养的星形胶质细胞中APP mrna均有不同比例的上调。与对照组相比，bFGF和EGF处理组的APP mrna分别增加了约4.5倍和2倍。含kpi亚型APP751和APP770 mRNA的升高幅度相对大于APP695亚型mRNA的升高幅度。我们的发现为星形胶质细胞参与导致阿尔茨海默病和唐氏综合征老年斑发展的一系列事件提供了进一步的证据。
ALL three major transcripts of beta-amyloid precursor protein (APP) were expressed in astrocytes. The ratio for APP695, APP751 and APP770 isoform mRNAs being 1:4:2. Treatment with bFGF and EGF produced upregulation of APP mRNAs in cultured astrocytes in varying proportions. In comparison with controls, increases in APP mRNAs in bFGF and EGF treated groups were about 4.5 and 2 fold, respectively. The elevation in mRNAs of KPI-containing isoforms APP751 and APP770 was relatively more than the elevation in mRNA of APP695 isoform. Our findings provide further evidence for the involvement of astrocytes in a cascade of events leading to the development of senile plaques in Alzheimer's disease and Down's syndrome.