An ELISA for detection of antibodies to the E2 protein of GB virus C.
An ELISA for detection of antibodies to the E2 protein of GB virus C.
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用于检测 GB 病毒 C 的 E2 蛋白抗体的 ELISA。
DOI：
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发表时间：
1997
影响因子：
6.4
通讯作者：
I. Mushahwar
中科院分区：
文献类型：
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作者：
B. Dille;T. Surowy;R. Gutierrez;P. Coleman;M. Knigge;R. Carrick;R. Aach;F. Hollinger;C. Stevens;L. Barbosa;G. Nemo;J. Mosley;G. Dawson;I. Mushahwar
利用CHO细胞表达的重组E2蛋白建立了检测GB病毒C（GBV-C）抗体的ELISA方法。在通过输血感染GBV-C RNA阳性血液的几个人中注意到血清转化为抗E2阳性。6例受血者感染GBV-C RNA后，4例（67%）抗E_2阳性，病毒血症清除。因此，抗E2血清转化与病毒清除相关。E2抗体在志愿献血者中的流行率相对较低（3.0%-8.1%），但在其他几个群体中较高，包括血浆置换献血者（34.0%）、静脉吸毒者（85.2%）和西非受试者（13.3%），所有这些人的GBV-C逆转录聚合酶链反应（RT-PCR）检测结果均为阴性。这些数据表明，抗E2的检测将大大扩展RT-PCR的能力，以确定GBV-C的流行病学和临床意义。
An ELISA was developed for detection of antibodies to GB virus C (GBV-C) using a recombinant E2 protein expressed in CHO cells. Seroconversion to anti-E2 positivity was noted among several persons infected with GBV-C RNA-positive blood through transfusion. Of 6 blood recipients infected by GBV-C RNA-positive donors, 4 (67%) became anti-E2 positive and cleared their viremia. Thus, anti-E2 seroconversion is associated with viral clearance. The prevalence of antibodies to E2 was relatively low (3.0%-8.1%) in volunteer blood donors but was higher in several other groups, including plasmapheresis donors (34.0%), intravenous drug users (85.2%), and West African subjects (13.3%), all of whom tested negative by GBV-C reverse-transcription polymerase chain reaction (RT-PCR). These data demonstrate that testing for anti-E2 should greatly extend the ability of RT-PCR to define the epidemiology and clinical significance of GBV-C.