Quantitative assessment of articular cartilage and subchondral bone histology in the meniscectomized guinea pig model of osteoarthritis
Quantitative assessment of articular cartilage and subchondral bone histology in the meniscectomized guinea pig model of osteoarthritis
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DOI：
10.1016/s1063-4584(03)00050-5
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发表时间：
2003-06-01
影响因子：
7
通讯作者：
De Ceuninck, F
中科院分区：
文献类型：
--
作者：
Pastoureau, P;Leduc, S;De Ceuninck, F
目的：采用一种新的图像分析系统，以量化的主要组织学参数反映骨关节炎的特点，在软骨和骨水平，在半月板切除豚鼠模型的骨关节炎（OA.Methods）：半月板切除（MNX）和假手术（SH）豚鼠进行了研究后1个月和3个月的部分半月板切除术在内侧的左膝（n = 10至12只动物/组）。左侧胫骨近端包含在甲基丙烯酸甲酯中。切片，用番红O或Goldner三色染色。使用Biocorn图像分析仪的特殊程序定量参数。在胫骨内侧评价以下参数：软骨厚度（CT）;纤维化指数（FI）;基于番红O染色强度（PC）的蛋白多糖含量比;软骨细胞密度（CD）;骨体积（BV）和软骨下骨板厚度（SBPT）。结果：半月板切除术后1个月，这些可重复的组织形态计量学参数均发生显著变化（FI：+522%，P
Objective: A new image analysis system was employed to quantify the main histological parameters reflecting osteoarthritic features, at the cartilage and bone levels, in the meniscectomized guinea pig model of osteoarthritis (OA).Methods: Meniscectomized (MNX) and sham-operated (SH) guinea pigs were studied 1 and 3 months after partial meniscectomy at the medial side of the left knee (n = 10 to 12 animals/group). The left proximal tibias were included in methylmethacrylate. Sections were cut and stained with safranin O or Goldner trichrome. Parameters were quantified using special programs of a Biocorn image analyser. The following parameters were evaluated at the medial side of the tibia: cartilage thickness (CT); fibrillation index (FI); proteoglycan content ratio based on safranin O staining intensities (PC); chondrocyte density (CD); bone volume (BV) and subchondral bone plate thickness (SBPT). The degree of user interaction varied from manually tracing objects to almost complete computer automation.Results: Meniscectomy resulted in significant variations of these reproducible histomorphometric parameters both after 1 month (FI: +522%, P