Keratinolytic activity of cutaneous and oral bacteria
Keratinolytic activity of cutaneous and oral bacteria
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皮肤和口腔细菌的角质分解活性
DOI：
10.1128/iai.55.3.621-625.1987
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发表时间：
1987
影响因子：
3.1
通讯作者：
M. De Jong
中科院分区：
文献类型：
--
作者：
F. Mikx;M. De Jong
建立了一种检测皮肤和口腔细菌角蛋白分解活性的方法。用异硫氰酸荧光素标记的角蛋白，在含有1 mM二硫苏糖醇的磷酸盐缓冲液(pH 7.2)中使用。通过在发光光谱仪中测量反应混合物上清液中降解产物的荧光来评估角蛋白的降解。调查了几种口腔和皮肤细菌：牙龈拟杆菌、中间类杆菌、齿密螺旋体、齿状放线菌、表皮葡萄球菌、溶血葡萄球菌、人葡萄球菌、腐生葡萄球菌、表皮短杆菌、短杆菌、谷氨酸棒杆菌、细小棒状杆菌、绵羊棒状杆菌和马红球菌。以皮肤真菌红色毛霉菌为对照。本试验提供了一种简便、定量的角蛋白降解方法，并使我们能够显示红色毛霉菌、表皮毛霉菌、溶血性毛霉菌、亚麻毛霉菌、牙龈拟杆菌和齿密螺旋体的角蛋白降解活性。角蛋白溶解活性具有细胞结合性和热敏性。二硫苏糖醇的存在刺激了角蛋白的降解，主要是高分子量的产物。
A test was developed to measure the keratinolytic activity of cutaneous and oral bacteria. Keratin, labeled with fluorescein isothiocyanate, was used in a phosphate buffer (pH 7.2) with 1 mM dithiothreitol. The degradation of keratin was estimated by measuring the fluorescence of the degradation products in the supernatant of the reaction mixtures in a luminescence spectrometer. Several oral and cutaneous bacteria were investigated: Bacteroides gingivalis, Bacteroides intermedius, Treponema denticola, Actinomyces odontolyticus, Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus hominis, Staphylococcus saprophyticus, Brevibacterium epidermidis, Brevibacterium lines, Corynebacterium glutamicum, Corynebacterium minutissimum, Corynebacterium ovis, and Rhodococcus equi. The dermatophyte Trichophyton rubrum was used as a control organism. The test offered a simple and quantitative method for the estimation of keratin degradation and enabled us to show keratinolytic activity in Trichophyton rubrum, S. epidermidis, S. haemolyticus, B. linens, B. epidermidis, Bacteroides gingivalis, and Treponema denticola. The keratinolytic activity was cell bound and heat sensitive. The presence of dithiothreitol stimulated the degradation of keratin to mainly high-molecular-weight products.