RESOLUTION OF METHAMPHETAMINE STEREOISOMERS IN URINE DRUG-TESTING - URINARY-EXCRETION OF R(-)-METHAMPHETAMINE FOLLOWING USE OF NASAL INHALERS
RESOLUTION OF METHAMPHETAMINE STEREOISOMERS IN URINE DRUG-TESTING - URINARY-EXCRETION OF R(-)-METHAMPHETAMINE FOLLOWING USE OF NASAL INHALERS
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DOI：
10.1093/jat/12.5.255
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发表时间：
1988-09-01
影响因子：
2.5
通讯作者：
POKLIS, A
中科院分区：
文献类型：
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作者：
FITZGERALD, RL;RAMOS, JM;POKLIS, A
本研究的目的是确定从鼻吸入器吸入的 R(-)-甲基苯丙胺在目前使用的尿液药物筛查程序中是否会产生阳性甲基苯丙胺结果，并提出一种快速区分甲基苯丙胺旋光异构体的方法。三名受试者在六小时内每 20 分钟从 Vicks 鼻吸入器吸入一次，经 EMIT、Toxilab、TDx 和 GC/MS 检测，其尿液中甲基苯丙胺呈阳性。手性衍生试剂 N-三氟乙酰基-L-脯氨酰氯 (L-TPC) 用于形成甲基苯丙胺非对映体，从而可以快速鉴定尿液样本中存在的甲基苯丙胺的每种立体异构体。在常规药物筛选过程中，尿液样本安非他明呈阳性，经确定由甲基安非他明的外消旋混合物组成。尿液样本中存在的甲基苯丙胺的异构体组成表明了该药物的可能来源。
The objective of this study is to determine whether R(-)-methamphetamine inhaled from nasal inhalers produces positive methamphetamine results in currently used urine drug screening procedures and to present a rapid method for distinguishing the optical isomers of methamphetamine. Urine from three subjects inhaling from a Vicks Nasal Inhaler every 20 min for six hours tested positive for methamphetamine by EMIT, Toxilab, TDx, and GC/MS. The chiral derivatizing reagent N-trifluoroacetyl-L-prolyl chloride (L-TPC) was used to form methamphetamine diastereomers allowing rapid identification of each stereoisomer of methamphetamine present in the urine samples. Urine samples positive for amphetamines during routine drug screening were determined to consist of a racemic mixture of methamphetamine. The isomeric composition of methamphetamine present in a urine sample indicates the probable source of the drug.