Pleiotrophin promotes functional recovery after neural transplantation in rats
Pleiotrophin promotes functional recovery after neural transplantation in rats
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DOI：
10.1097/wnr.0b013e328011398e
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发表时间：
2007-01-22
期刊：
影响因子：
1.7
通讯作者：
Nishino, Hitoo
中科院分区：
文献类型：
--
作者：
Hida, Hideki;Masuda, Tadashi;Nishino, Hitoo
多效蛋白在体外促进多巴胺能神经元的存活。为了研究多效素是否促进体内移植的多巴胺能神经元的存活，在细胞制备过程中用多效素（100 ng/ml）处理来自腹侧中脑的供体细胞，并将其移植到半帕金森模型大鼠的纹状体中。在多效素治疗组中，甲基苯丙胺诱导的旋转的功能恢复得到改善，更多的酪氨酸羟化酶阳性细胞在纹状体中存活。在移植前向细胞中添加多效素也能带来更好的功能恢复。然而，在细胞制备过程中未观察到 caspase-3 激活。有趣的是，多效蛋白对存活的影响与神经胶质细胞系衍生的中性粒细胞因子的影响相加。这些结果表明多效蛋白对体内神经移植中的供体细胞有影响。
Pleiotrophin promotes survival of dopaminergic neurons in vitro. To investigate whether pleiotrophin promotes survival of grafted dopaminergic neurons in vivo, donor cells from ventral mesencepha-Ion were treated with pleiotrophin (100 ng/ml) during cell preparation and grafted into striatum of hemi-Parkinson model rats. Functional recovery in methamphetamine-induced rotations was improved, and more tyrosine hydroxylase-positive cells survived in the striatum in the pleiotrophin-treated group. Pleiotrophin addition to cells just before transplantation also resulted in better functional recovery; however, no caspase-3 activation was seen during cell preparation. Interestingly, the effect of pleiotrophin on the survival was additive to that of glial-cell line-derived neutropic factor. These results revealed that pleiotrophin had effects on donor cells in neural transplantation in vivo.