Proteomic analysis of apoptotic and oncotic pancreatic acinar AR42J cells treated with caerulein
Proteomic analysis of apoptotic and oncotic pancreatic acinar AR42J cells treated with caerulein
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DOI：
10.1007/s11010-013-1603-0
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发表时间：
2013-10-01
影响因子：
4.3
通讯作者：
Xue, Dongbo
中科院分区：
文献类型：
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作者：
Chu, Jiangtao;Ji, Hongliang;Xue, Dongbo
本研究旨在检测雨蛙肽诱导胰腺腺泡细胞凋亡和胀亡过程中差异表达的蛋白质，探讨腺泡细胞不同的死亡过程。用雨蛙肽处理AR 42 J细胞，诱导急性胰腺炎细胞模型。通过流式细胞术分离正在经历凋亡和胀亡的细胞。采用鸟枪液相色谱-串联质谱法检测两组分离细胞中差异表达的蛋白质。结果显示，凋亡组和胀亡组均检测到11种蛋白，凋亡组仅检测到17种蛋白，胀亡组仅检测到29种蛋白。KEGG分析显示，仅凋亡组蛋白质在ECM-受体相互作用、细胞粘附分子等10条途径中显著富集，而仅胀亡组蛋白质在胞吞、碱基切除修复和RNA降解3条途径中显著富集。这些蛋白质的检测有助于我们了解急性胰腺炎细胞死亡的过程，并可能有助于改变胰腺腺泡细胞的死亡方式，从而减轻胰腺炎的严重程度。
This study aims to determine the differentially expressed proteins in the pancreatic acinar cells undergoing apoptosis and oncosis stimulated with caerulein to explore different cell death process of the acinar cell. AR42J cells were treated with caerulein to induce cell model of acute pancreatitis. Cells that were undergoing apoptosis and oncosis were separated by flow cytometry. Then differentially expressed proteins in the two groups of separated cells were detected by shotgun liquid chromatography-tandem mass spectrometry. The results showed that 11 proteins were detected in both apoptosis group and oncosis group, 17 proteins were detected only in apoptosis group and 29 proteins were detected only in oncosis group. KEGG analysis showed that proteins detected only in apoptosis group were significantly enriched in 10 pathways, including ECM-receptor interaction, cell adhesion molecules, and proteins detected only in oncosis group were significantly enriched in three pathways, including endocytosis, base excision repair, and RNA degradation. These proteins we detected are helpful for us to understand the process of cell death in acute pancreatitis and may be useful for changing the death mode of pancreatic acinar cells, thus attenuating the severity of pancreatitis.