The integrity of PRRSV nucleocapsid protein is necessary for up-regulation of optimal interleukin-10 through NF-κB and p38 MAPK pathways in porcine alveolar macrophages
The integrity of PRRSV nucleocapsid protein is necessary for up-regulation of optimal interleukin-10 through NF-κB and p38 MAPK pathways in porcine alveolar macrophages
复制标题
PRRSV 核衣壳蛋白的完整性对于通过猪肺泡巨噬细胞中的 NF-kappa B 和 p38 MAPK 途径上调最佳白细胞介素 10 是必要的
DOI：
10.1016/j.micpath.2017.04.036
复制
发表时间：
2017-08-01
影响因子：
3.8
通讯作者：
Wang, Jinbao
中科院分区：
文献类型：
--
作者：
Yu, Jiang;Liu, Yanyan;Wang, Jinbao
猪繁殖与呼吸综合征（PRRS）是一种高度传染性疾病，在世界范围内不断造成巨大的经济损失。PRRS病毒（PRRSV）感染导致免疫抑制和IL-10上调。他们之间的关系仍有争议。以往的研究表明PRRSV核衣壳蛋白(N)蛋白能够上调IL-10，但其潜在的分子机制尚不清楚。本研究分析了PRRSV N蛋白诱导IL-10上调在永活猪肺泡巨噬细胞（PAM5）中的表达动力学。N蛋白诱导IL-10表达呈时间和剂量依赖性。信号通路抑制实验提示NF-kappa B和p38 MAPK通路均参与N蛋白诱导的IL-10上调。此外，N蛋白的完整性对IL-10的显著上调至关重要。本研究有助于进一步了解PRRSV与宿主免疫系统的相互作用。(C) 2017年Elsevier Ltd.出版
Porcine reproductive and respiratory syndrome (PRRS), a highly contagious disease, has been constantly causing huge economic losses all over the world. PRRS virus (PRRSV) infection results in immunosuppression and IL-10 up-regulation. The relationship between them is still in dispute. Previous studies demonstrated the protein of PRRSV nucleocapsid (N) protein is able to up-regulate IL-10, yet the underlying molecular mechanisms remain unknown. In this study, the expression kinetics of IL-10 up regulation induced by PRRSV N protein were analyzed in immortalized porcine alveolar macrophages (PAM5). N protein induced IL-10 expression in a time- and dose-dependent manner. Inhibition experiments of signaling pathways suggested NF-kappa B and p38 MAPK pathways are both involved in N protein induced IL-10 up-regulation. Besides, the integrity of N protein is essential for significant IL-10 up regulation. This research is beneficial for further understanding of the interplay between PRRSV and host immune system.(C) 2017 Published by Elsevier Ltd.