Coelenterazine Analogue with Human Serum Albumin-Specific Bioluminescence
Coelenterazine Analogue with Human Serum Albumin-Specific Bioluminescence
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DOI：
10.1021/acs.bioconjchem.0c00536
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发表时间：
2020-12-16
影响因子：
4.7
通讯作者：
Kurita, Ryoji
中科院分区：
文献类型：
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作者：
Nishihara, Ryo;Niwa, Kazuki;Kurita, Ryoji
以咪唑吡嗪酮为核心合成荧光素HuLumino1，在真实血清样品中与人血清白蛋白（HSA）产生特异性生物发光。HuLumino1是通过在coelenterazine的C-6上连接甲氧基端烷基链，并在C-8上消去苄基而得到的。由于HuLumino1的高特异性，在不需要任何样品预处理的情况下，酶联免疫吸附测定的HSA水平在5%的误差范围内被量化。
A synthetic luciferin comprising an imidazopyrazinone core, named HuLumino1, was designed to generate specific bioluminescence with human serum albumin (HSA) in real serum samples. HuLumino1 was developed by attaching a methoxy-terminated alkyl chain to C-6 of coelenterazine and by eliminating a benzyl group at C-8. HSA levels were quantified within 5% error margins of an enzyme-linked immunosorbent assay without the need for any sample pretreatments because of the high specificity of HuLumino1.