Genomic and molecular characterization of a novel quorum sensing molecule in Bacillus licheniformis.
Genomic and molecular characterization of a novel quorum sensing molecule in Bacillus licheniformis.
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DOI：
10.1186/s13568-017-0381-6
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发表时间：
2017-12
期刊：
影响因子：
3.7
通讯作者：
Keshavarz T
中科院分区：
文献类型：
--
作者：
Esmaeilishirazifard E;De Vizio D;Moschos SA;Keshavarz T
群体感应分子（QSM）参与复杂过程的调节，帮助细菌群体对其细胞密度的变化做出反应。虽然QS基因簇（comQXPA）已在一些杆菌的基因组序列中被鉴定出来，但地衣芽孢杆菌的QS系统尚未被详细研究，其QSM（ComX信息素）也尚未被鉴定。鉴于这种拮抗细菌作为工业主力的重要性，本研究旨在阐明地衣芽孢杆菌 NCIMB-8874 QS。对该菌株全基因组序列的生物信息学研究结果证实了必需的群体感应相关基因的存在。尽管在该簇的三个蛋白质 ComQ、前体 ComX 和 ComP 中验证了多态性，但转录因子 ComA 被确认为最保守的蛋白质。通过进一步阐明 13 个氨基酸肽的 ComX 信息素，研究了地衣芽孢杆菌 NCIMB-8874 的细胞间通讯。信息素的肽序列已通过生化表征进行了描述。本文的在线版本 (doi:10.1186/s13568-017-0381-6) 包含补充材料，可供授权用户使用。
Quorum sensing molecules (QSMs) are involved in the regulation of complicated processes helping bacterial populations respond to changes in their cell-density. Although the QS gene cluster (comQXPA) has been identified in the genome sequence of some bacilli, the QS system B. licheniformis has not been investigated in detail, and its QSM (ComX pheromone) has not been identified. Given the importance of this antagonistic bacterium as an industrial workhorse, this study was aimed to elucidate B. licheniformis NCIMB-8874 QS. The results obtained from bioinformatics studies on the whole genome sequence of this strain confirmed the presence of essential quorum sensing-related genes. Although polymorphism was verified in three proteins of this cluster, ComQ, precursor-ComX and ComP, the transcription factor ComA was confirmed as the most conserved protein. The cell–cell communication of B. licheniformis NCIMB-8874 was investigated through further elucidation of the ComX pheromone as 13-amino acid peptide. The peptide sequence of the pheromone has been described through biochemical characterisation. The online version of this article (doi:10.1186/s13568-017-0381-6) contains supplementary material, which is available to authorized users.