RNA sequencing of microglia and monocyte-derived macrophages from mice with experimental autoimmune encephalomyelitis illustrates a changing phenotype with disease course
RNA sequencing of microglia and monocyte-derived macrophages from mice with experimental autoimmune encephalomyelitis illustrates a changing phenotype with disease course
复制标题
DOI：
10.1016/j.jneuroim.2014.09.014
复制
发表时间：
2014-12-15
影响因子：
3.3
通讯作者：
Modis, Louise K.
中科院分区：
文献类型：
--
作者：
Lewis, Nuruddeen D.;Hill, Jonathan D.;Modis, Louise K.
小胶质细胞和单核细胞衍生的巨噬细胞在实验性自身免疫性脑脊髓炎发病机制中的作用一直存在争议。为了深入了解它们各自的作用，我们开发了一种利用抗CD 44抗体通过流式细胞术区分CNS中小胶质细胞和单核细胞衍生的巨噬细胞的方法。我们使用该系统通过RNA测序来监测疾病过程中细胞数量、激活状态和基因表达的变化。这种体内表征和RNA-Seq数据集提高了我们对炎症条件下大脑中巨噬细胞生物学的理解，并可能导致识别神经炎性疾病疗法的策略。(C)2014爱思唯尔有限公司版权所有。
The role of microglia and monocyte-derived macrophages in experimental autoimmune encephalomyelitis pathogenesis has been controversial. To gain insight into their respective roles, we developed a method for differentiating between microglia and monocyte-derived macrophages in the CNS by flow cytometry utilizing anti-CD44 antibodies. We used this system to monitor changes in cell number, activation status, and gene expression by RNA sequencing over the course of disease. This in vivo characterization and RNA-Seq dataset improves our understanding of macrophage biology in the brain under inflammatory conditions and may lead to strategies to identify therapies for neuroinflammatory diseases. (C) 2014 Elsevier B.V. All rights reserved.