Stearoyl‐CoA desaturase and carotenoids concertedly enhance the resistance of pearl oyster Pinctada fucata to high temperature stress
Stearoyl‐CoA desaturase and carotenoids concertedly enhance the resistance of pearl oyster Pinctada fucata to high temperature stress
复制标题
硬脂酰辅酶A去饱和酶和类胡萝卜素协同增强珠母贝对高温胁迫的抵抗力
DOI：
10.1111/are.14835
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发表时间：
2020
影响因子：
2
通讯作者：
Dahui Yu
中科院分区：
文献类型：
--
作者：
Lirong Bai;Zihao Meng;Baosuo Liu;Deguo Yang;Dahui Yu
我们鉴定了合浦珠母贝（Pinctada fucata）中一个新的硬脂酰辅酶A去饱和酶基因PfSCD，并研究了其在高、低类胡萝卜素含量（橙子和白色组）的不同组织中的表达水平以及对高温胁迫（26°C ~ 30°C）的响应。结果表明，合浦珠母贝cDNA全长1,576 bp，开放阅读框945 bp，编码315个氨基酸，包含4个跨膜结构域和3个保守的组氨酸盒结构域。与白色组相比，橙子组的PfSCD mRNA相对表达量、总类胡萝卜素含量（TCC）和总抗氧化能力（TAC）均显著上调，其中肝胰腺最高，性腺次之，足最低。在30°C胁迫0 ~ 96 h期间，橙子组PfSCD相对表达量先升高后降低，在6 h和12 h达到峰值。同时，TCC和TAC在应激过程中逐渐下降，橙子组肝胰腺和性腺组织中TCC和TAC在6 h时分别显著升高，对应于6 h时PfSCD的升高。这表明PfSCD可以上调类胡萝卜素。高温胁迫下PfSCDmRNA与TCC和TAC的正相关性均被破坏。这些结果表明，SCD和类胡萝卜素可以相互上调，协同提高牡蛎对高温胁迫的抵抗力。
We characterized a novel stearoyl‐CoA desaturase gene fromPinctada fucata, namedPfSCD, and investigated the expression levels in various tissues of high and low carotenoid contents (orange and white groups) and the responses to high temperature stress (from 26°C to 30°C). The complete cDNA sequence consisted of 1,576 bp with a 945 bp open reading frame encoding 315 amino acid residues; it included four transmembrane domains and three conserved histidine‐box motifs.Pinctada fucatawas clustered with molluscanSCDgenes. The relative expression level ofPfSCDmRNA, total carotenoid content (TCC) and total antioxidant capacity (TAC) was greatly up‐regulated in orange group as compared with the white, with the highest in hepatopancreas and followed by gonad tissue and the lowest in foot. The relative expression level ofPfSCDincreased first and then decreased during the stress period of temperature 30°C from 0 to 96 hr in the orange group with the peaks at 6 and 12 hr. Meanwhile, TCC and TAC decreased gradually during the stress, with a significant increase at 6 hr in the hepatopancreas and gonadal tissues, respectively, in the orange group, corresponding to the rise ofPfSCDat 6 hr. It suggested that carotenoids can be up‐regulated byPfSCD. Moreover, the positive correlations ofPfSCDmRNA with TCC and TAC were both disrupted under the high temperature stress. These findings suggested thatSCDand carotenoids can be up‐regulated by each other and concertedly enhance the resistance of the oyster to high temperature stress.