Co-production of vascular endothelial cadherin and inducible nitric oxide synthase by endothelial cells in periapical granuloma
Co-production of vascular endothelial cadherin and inducible nitric oxide synthase by endothelial cells in periapical granuloma
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DOI：
10.1111/j.1365-2591.2006.01068.x
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发表时间：
2006-03-01
影响因子：
5
通讯作者：
Ito, K
中科院分区：
文献类型：
--
作者：
Hama, S;Takeichi, O;Ito, K
目的通过检测根尖周肉芽肿组织中血管内皮钙粘蛋白（VE）和诱导型一氧化氮合酶（iNOS）的表达，探讨根尖周肉芽肿组织中炎性细胞迁移的机制。用丙酮或4%多聚甲醛磷酸盐缓冲液固定组织后，分别制备5 μ m厚的石蜡或低温恒温切片。用苏木精-伊红染色对发炎组织的石蜡切片进行组织学评价。诊断为根尖周肉芽肿的组织的冷冻切片，然后通过使用抗人VE-钙粘蛋白或iNOS抗体（Abs）的免疫组织化学检查浸润细胞的表征。此外，还通过双色免疫荧光图像分析来分析VE-cadherin和iNOS产生的共定位。巨噬细胞、淋巴细胞、多形核白细胞和成纤维细胞也表现出iNOS的产生。这些iNOS阳性细胞聚集在血管周围。另一方面，VE-钙粘蛋白的产生仅在内皮细胞中表现出。结论在根尖肉芽肿中，内皮细胞产生的血管内皮钙粘素（Vascularendothelialcadherin，VEC-cadherin）可能受iNOS产生细胞的调节，在根尖肉芽肿的血管通透性中起重要作用。
Aim To clarify the mechanisms of inflammatory cell migration in human periapical granulomas by examining vascular endothelial (VE) cadherin and inducible nitric oxide synthase (iNOS)-producing cells.Methodology Periapical tissues were obtained from patients during endodontic surgery and were divided into two portions. After fixing the tissues with acetone or 4% paraformaldehyde in phosphate-buffered saline, 5-mu m-thick paraffin or cryostat sections were prepared, respectively. The paraffin sections of the inflamed tissues were evaluated histologically with haematoxylin-eosin stains. Cryostat sections of the tissue, diagnosed as periapical granulomas, were then examined by either immunohistochemistry using anti-human VE-cadherin or iNOS antibodies (Abs) for the characterization of infiltrating cells. In addition, co-localization of VE-cadherin and iNOS production was also analysed by two-colour immunofluorescence image analysis.Results Endothelial cells were strongly stained with iNOS Abs. Macrophages, lymphocytes, polymorphonuclear leucocytes and fibroblasts also exhibited iNOS production. These iNOS-positive cells accumulated around the blood vessels. On the other hand, VE-cadherin production was exhibited in only endothelial cells. Two-colour immunofluorescence image analysis using VE-cadherin and iNOS Abs demonstrated that iNOS-producing endothelial cells also showed VE-cadherin production.Conclusions Vascular endothelial-cadherin produced by endothelial cells could be regulated by iNOS-producing cells in periapical granulomas and might play a pivotal role in vascular permeability.