ESTIMATION OF HYDROGEN-PEROXIDE IN PLANT-EXTRACTS USING TITANIUM(IV)
ESTIMATION OF HYDROGEN-PEROXIDE IN PLANT-EXTRACTS USING TITANIUM(IV)
复制标题
DOI：
10.1016/0003-2697(84)90039-3
复制
发表时间：
1984-01-01
影响因子：
2.9
通讯作者：
FERGUSON, IB
中科院分区：
文献类型：
--
作者：
PATTERSON, BD;MACRAE, EA;FERGUSON, IB
使用钛（IV）的丙酮提取物中的过氧化氢的估计方法可能会高估过氧化氢时，适用于植物叶片。颜料显然与Ti络合物共沉淀，并且不能通过用溶剂洗涤除去。氟化物专门去除钛-过氧化物复合物的颜色，仅去除与植物提取物反应产生的部分颜色。用三氯乙酸提取组织，并通过其与Ti（IV）与4-（2-吡啶偶氮）间苯二酚的络合物的反应来测量过氧化氢酶处理的空白的过氧化物，从而避免了这个问题。各种物种的叶中的过氧化氢水平范围为约100-约1000。0.1-0.6 μ mol·cm-1。G-1.
Methods for the estimation of hydrogen peroxide in acetone extracts using Ti(IV) are likely to overestimate hydrogen peroxide when applied to plant leaves. Pigments apparently co-precipitate with the Ti complex and cannot be removed by washing with solvents. Fluoride, which specifically removes the color of the Ti-peroxide complex, removes only some of the color from the reactions with plant extracts. This problem was avoided by extracting tissues with trichloroacetic acid, and measuring peroxide against catalase-treated blanks by its reaction with the complex of Ti(IV) with 4-(2-pyridylazo)resorcinol. Levels of hydrogen peroxide in leaves of a variety of species ranged from .apprx. 0.1-0.6 .mu.mol .cntdot. g-1.