Highly Bright AIE Nanoparticles by Regulating the Substituent of Rhodanine for Precise Early Detection of Atherosclerosis and Drug Screening
Highly Bright AIE Nanoparticles by Regulating the Substituent of Rhodanine for Precise Early Detection of Atherosclerosis and Drug Screening
复制标题
通过调节绕丹宁取代基的高亮度AIE纳米粒子用于动脉粥样硬化的精确早期检测和药物筛选。
DOI：
10.1002/adma.202106994
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发表时间：
2022-01-23
影响因子：
29.4
通讯作者：
Ding，Dan
中科院分区：
文献类型：
--
作者：
Wang，Kai;Gao，Heqi;Ding，Dan
能够在早期阶段精确检测动脉粥样硬化（AS）和在动物水平上快速评估抗AS药物的荧光探针特别有价值。在此，通过调节罗丹宁的取代基引入高亮度聚集诱导发射（AIE）纳米探针，用于早期检测动脉粥样硬化斑块和以精确、灵敏和快速的方式筛选抗AS药物。以二氰基亚甲基取代的若丹宁为吸电子单元，与对照化合物相比，AIE发光体TPE-T-RCN表现出最高的摩尔消光系数、最大的光致发光量子产率和最大的吸收/发射光谱红移。纳米探针是用两亲性共聚物作为包封TPE-T-RCN分子的基质获得的，所述TPE-T-RCN分子进一步用抗CD 47抗体进行表面官能化，以特异性结合AS斑块中过表达的CD 47。这种纳米探针可以有效识别载脂蛋白E缺陷（apoE−/−）小鼠不同阶段的AS斑块，特别是在微计算机断层扫描（CT）和磁共振成像（MRI）之前识别早期AS斑块。这些特点促使纳米探针应用于抗AS药物疗效监测，为抗AS药物筛选提供了有力的工具。它们在人颈动脉斑块靶向成像中的潜在用途得到进一步证实。
Fluorescent probes capable of precise detection of atherosclerosis (AS) at an early stage and fast assessment of anti‐AS drugs in animal level are particularly valuable. Herein, a highly bright aggregation‐induced emission (AIE) nanoprobe is introduced by regulating the substituent of rhodanine for early detection of atherosclerotic plaque and screening of anti‐AS drugs in a precise, sensitive, and rapid manner. With dicyanomethylene‐substituted rhodanine as the electron‐withdrawing unit, the AIE luminogen named TPE‐T‐RCN shows the highest molar extinction coefficient, the largest photoluminescence quantum yield, and the most redshifted absorption/emission spectra simultaneously as compared to the control compounds. The nanoprobes are obtained with an amphiphilic copolymer as the matrix encapsulating TPE‐T‐RCN molecules, which are further surface functionalized with anti‐CD47 antibody for specifically binding to CD47 overexpressed in AS plaques. Such nanoprobes allow efficient recognition of AS plaques at different stages in apolipoprotein E‐deficient (apoE−/−) mice, especially for the recognition of early‐stage AS plaques prior to micro‐computed tomography (CT) and magnetic resonance imaging (MRI). These features impel to apply the nanoprobes in monitoring the therapeutic effects of anti‐AS drugs, providing a powerful tool for anti‐AS drug screening. Their potential use in targeted imaging of human carotid plaque is further demonstrated.