Clinical significance of zinc finger E-box binding homeobox 1 (ZEB1) in human gastric cancer
Clinical significance of zinc finger E-box binding homeobox 1 (ZEB1) in human gastric cancer
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DOI：
10.1002/jso.22142
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发表时间：
2012-09-01
影响因子：
2.5
通讯作者：
Kusunoki, Masato
中科院分区：
文献类型：
--
作者：
Okugawa, Yoshinaga;Toiyama, Yuji;Kusunoki, Masato
背景和目的 锌指 E 盒结合同源盒 1 (ZEB1) 编码转录因子，是上皮间质转化 (EMT) 诱导基因之一，在各种癌症的肿瘤进展中发挥关键作用。本研究的目的是阐明ZEB1在胃癌患者中表达的临床意义。方法 对 134 名接受胃癌手术的患者进行评估。我们通过实时逆转录 PCR 分析了胃癌组织和邻近正常粘膜中的 ZEB1 mRNA 水平。使用免疫组织化学分析测量原发性癌症和腹膜播散样本中的ZEB1蛋白表达。结果ZEB1基因在癌组织中的表达显着高于癌旁正常粘膜。 ZEB1表达增加与腹膜播散显着相关，并且是一个独立的预后因素。 Logistic回归分析显示ZEB1表达增加是腹膜播散的独立危险因素。免疫组织化学分析表明，ZEB1 在原发性癌症和腹膜播散样本中均强烈表达。结论 ZEB1是胃癌腹膜播散的独立因素，可能在胃癌腹膜播散进展中发挥关键作用。 J.外科医生。安科尔。 2012年； 106：280-285。 (c) 2011 年 Wiley 期刊公司。
Background and Objectives Zinc finger E-box Binding homeobox 1 (ZEB1) encodes a transcription factor and is one of the epithelialmesenchymal transition (EMT)-inducible genes that play a key role in tumor progression in various cancers. The aim of this study is to clarify the clinical significance of ZEB1 expression in gastric cancer patients. Methods One hundred thirty-four patients who underwent surgery for gastric cancer were evaluated. We analyzed ZEB1 mRNA levels by real-time reverse transcription PCR in gastric cancer tissue and adjacent normal mucosa. ZEB1 protein expression in primary cancer and in peritoneal dissemination samples was measured using immunohistochemical analysis. Results Expression of the ZEB1 gene was significantly higher in cancerous tissue than in adjacent normal mucosa. Increased ZEB1 expression was significantly associated with peritoneal dissemination, and was an independent prognostic factor. Logistic regression analysis revealed that increased ZEB1 expression was an independent risk factor for peritoneal dissemination. Immunohistochemical analysis indicated that ZEB1 was intensely expressed in both primary cancer and peritoneal dissemination samples. Conclusions ZEB1 is an independent factor for peritoneal dissemination in patients with gastric cancer, and may therefore play a key role in the progression to peritoneal dissemination in gastric cancer patients. J. Surg. Oncol. 2012; 106:280-285. (c) 2011 Wiley Periodicals, Inc.