Silicon Fertilizer and Microbial Agents Changed the Bacterial Community in the Consecutive Replant Soil of Lilies
Silicon Fertilizer and Microbial Agents Changed the Bacterial Community in the Consecutive Replant Soil of Lilies
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硅肥和微生物菌剂改变了百合连作土壤中的细菌群落
DOI：
10.3390/agronomy12071530
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发表时间：
2022-06
期刊：
影响因子：
--
通讯作者：
Guiying Shi
中科院分区：
文献类型：
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作者：
Yanlin Yu;Lipeng Zhang;Yuanpeng Li;Lei Hou;Hongyu Yang;Guiying Shi
摘要：作物复种导致土壤退化，土壤生产力下降，是农业可持续发展面临的挑战。我们之前发现，硅肥配合附加微生物剂是缓解各种中国百合在再植过程中出现的问题的有效手段，但对这一过程中微生物结构的变化知之甚少。在本研究中，采用对照CK、硅肥SF、微生物剂MF和硅肥微生物剂组合SMF 4种处理。我们连续3年对土壤进行处理，利用16S rRNA高通量测序技术对百合根区土壤细菌群落结构和一些特定微生物群进行了研究。结果表明，硅肥和微生物剂处理显著改善了植物的生长状况，改变了土壤细菌群落的多样性和结构。SF处理下假单胞菌属数量显著增加，SMF处理下放线菌门数量以及Nordella、Devosia和Rhodoplanes属数量显著增加。Nordella属、Pedomicrobium属和Chthoniobacter属与幼苗指数或有效硅含量相关。盖氏菌属和诺卡菌属是连接土壤细菌群落的关键种。土壤理化性质对土壤细菌群落结构的恢复起着重要作用。综上所述，硅肥和微生物剂改变了细菌群落的多样性和结构。在补肥模式下，放线菌门和假单胞菌属的富集对改善百合土壤健康和缓解CRPs起着重要作用。此外，有机质、速效磷、速效钾和速效硅是影响细菌群落结构恢复的最重要因素。
Abstract: Crop replanting leads to soil degradation and soil productivity reduction, which is a challenge for sustainable agricultural development. We previously found that silicon fertilizers combined with additional microbial agents are an effective means to alleviate problems that occur in a variety of Chinese lily during replanting, but little is known about the changes in microbial structure during this process. In the present study, we applied four treatments: CK (control), SF (silicon fertilizer), MF (microbial agents), and SMF (combination of silicon fertilizer and microbial agents). We treated the soil constantly for three years and investigated the bacterial community structure and some specific microbial groups in the soil of the lily root zone using 16S rRNA high throughput sequencing analysis. The results showed that silicon fertilizer and microbial agent treatment significantly improved the growth status of the plants and changed the diversity and structure of the bacterial community in the soil. The genus Pseudomonas significantly increased in the SF treatment, and the phylum Actinobacteria and the genera Nordella, Devosia, and Rhodoplanes significantly increased in the SMF treatment. The genera Nordella, Pedomicrobium, and Chthoniobacter correlated with the seedling index or available silicon content. In addition, the two genera Gaiella and Nocardioides were the key species linking the bacterial community in the soil. The soil physicochemical properties played an important role in restoring the soil bacterial community structures. In conclusion, silicon fertilizer and microbial agents changed the diversity and structure of the bacterial community. Under the fertilizer supplement model, the enrichment of the phylum Actinobacteria and the genus Pseudomonas played an important role in improving soil health and alleviating CRPs in lilies. In addition, organic matter, available phosphorus, available potassium, and available silicon were found to be the most important factors that have a great impact on the restoration of bacterial community structures.