CAP5.5, a life-cycle-regulated, cytoskeleton-associated protein is a member of a novel family of calpain-related proteins in Trypanosoma brucei
CAP5.5, a life-cycle-regulated, cytoskeleton-associated protein is a member of a novel family of calpain-related proteins in Trypanosoma brucei
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DOI：
10.1016/s0166-6851(01)00296-1
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发表时间：
2001-08-01
影响因子：
1.5
通讯作者：
Gull, K
中科院分区：
文献类型：
--
作者：
Hertz-Fowler, C;Ersfeld, K;Gull, K
非洲锥虫的细胞形状由广泛的膜下微管细胞骨架的存在决定。细胞骨架的其他可能的功能，如提供一个潜在的框架信号蛋白转导信息的细胞内和细胞外环境，尚未在锥虫进行了研究。在这项研究中，我们已经确定了一种新的细胞因子相关蛋白在布氏锥虫。CAP5.5是锥虫中一个新的蛋白质家族的第一个成员，其特征在于它们与钙蛋白酶型蛋白酶的催化区域相似。CAP5.5仅在原环锥虫中表达，而在血流锥虫中不表达。此外，CAP 5.5已被证明是肉豆蔻酰化和棕榈酰化的，表明与细胞膜的稳定相互作用。对锥虫基因组的生物信息学分析揭示了一个不同的钙蛋白酶相关蛋白家族，其一级结构与CAP5.5相似，但长度不同。我们建议在T.布鲁塞。(C)2001 Elsevier Science B.V.保留所有权利。
The cell shape of African trypanosomes is determined by the presence of an extensive subpellicular microtubule cytoskeleton. Other possible functions of the cytoskeleton, such as providing a potential framework for signalling proteins transducing information from the intracellular and extracellular environment, have not yet been investigated in trypanosomes. In this study, we have identified a novel cytoskeleton-associated protein in Trypanosoma brucei. CAP5.5 is the first member of a new family of proteins in trypanosomes, characterised by their similarity to the catalytic region of calpain-type proteases. CAP5.5 is only expressed in procyclic, but not in bloodstream, trypanosomes. Furthermore, CAP5.5 has been shown to be both myristoylated and palmitoylated, suggesting a stable interaction with the cell membrane. A bioinformatics analysis of the trypanosome genome revealed a diverse family of calpain-related proteins with primary structures similar to CAP5.5, but of varying length. We suggest a nomenclature for this new family of proteins in T. brucei. (C) 2001 Elsevier Science B.V. All rights reserved.