Fluorescence spectroscopy as a highly potential single-entity tool to identify chromophores and fluorophores: study on neoplastic human brain lesions
Fluorescence spectroscopy as a highly potential single-entity tool to identify chromophores and fluorophores: study on neoplastic human brain lesions
复制标题
DOI：
10.1117/1.jbo.18.6.067002
复制
发表时间：
2013-06-01
影响因子：
3.5
通讯作者：
Jayasree, Ramapurath S.
中科院分区：
文献类型：
--
作者：
Nazeer, Shaiju S.;Saraswathy, Ariya;Jayasree, Ramapurath S.
荧光和漫反射光谱是基于内源荧光团的发射和血红蛋白等吸收体的漫反射来区分正常组织和恶性组织的强大工具。然而，荧光团和血红蛋白的鉴定采用不同的分析方法。据报道，使用单一的自发荧光光谱技术同时估计荧光团和血红蛋白，并且潜在地开发了其对临床组织样本的诊断潜力。研究人员对手术切除的患者脑组织进行了研究，这些脑组织随后在病理学上被鉴定为星形细胞瘤、神经胶质瘤、脑膜瘤和神经鞘瘤。在 320 和 410 nm 激发波长下分析主要荧光团胶原蛋白、黄素腺嘌呤二核苷酸、磷脂和卟啉的发射。血红蛋白浓度还可根据 500 和 570 nm 处的荧光发射比率计算。当使用基于线性判别分析的诊断算法时，与 320 nm 激发相比，410 nm 激发可以更好地对正常组织和肿瘤组织进行分类。强调了荧光光谱作为单一实体评估正常和肿瘤脑组织内突出荧光团以及血红蛋白浓度的潜力。 (C) 2013 年光电仪器工程师学会 (SPIE)
Fluorescence and diffuse reflectance spectroscopy are powerful tools to differentiate normal and malignant tissue based on the emissions from endogenous fluorophores and diffuse reflection of absorbers such as hemoglobin. However, separate analytical methods are used for the identification of fluorophores and hemoglobin. The estimation of fluorophores and hemoglobin simultaneously using a single technique of autofluorescence spectroscopy is reported, and its diagnostic potential on clinical tissue samples is potentially exploited. Surgically removed brain tissues from patients that are later identified pathologically as astrocytoma, glioma, meningioma, and schwannoma are studied. The emissions from prominent fluorophores collagen, flavin adenine dinucleotide, phospholipids, and porphyrin are analyzed at 320 and 410 nm excitations. The hemoglobin concentration is also calculated from the ratio of fluorescence emissions at 500 and 570 nm. A better classification of normal and tumor tissues is yielded for 410 nm excitation compared to 320 nm when diagnostic algorithm based on linear discriminant analysis is used. The potential of fluorescence spectroscopy as a single entity to evaluate the prominent fluorophores as well as the hemoglobin concentration within normal and tumor brain tissues is emphasized. (C) 2013 Society of Photo-Optical Instrumentation Engineers (SPIE)