Wisteria Floribunda Agglutinin-Labeled Perineuronal Nets in the Mouse Inferior Colliculus, Thalamic Reticular Nucleus and Auditory Cortex.
Wisteria Floribunda Agglutinin-Labeled Perineuronal Nets in the Mouse Inferior Colliculus, Thalamic Reticular Nucleus and Auditory Cortex.
复制标题
DOI：
10.3390/brainsci6020013
复制
发表时间：
2016-04-13
期刊：
影响因子：
3.3
通讯作者：
Lee CC
中科院分区：
文献类型：
--
作者：
Fader SM;Imaizumi K;Yanagawa Y;Lee CC
神经周围网（PNN）是一种特殊的细胞外基质分子，与关键期的结束以及其他功能相关。在成人大脑中，PNN 围绕特定类型的神经元，然而 PNN 在小鼠听觉系统中的表达，特别是在中脑和前脑水平，尚未得到充分描述。此外，PNN 与这些结构中的兴奋性和抑制性细胞类型的关联仍然未知。因此，我们试图通过紫藤凝集素（WFA）标记来研究 PNN 在小鼠大脑下丘（IC）、丘脑网状核（TRN）和初级听觉皮层（A1）中的表达。为了帮助识别这些结构中的抑制性神经元，我们采用了囊泡 GABA 转运蛋白 (VGAT)-Venus 转基因小鼠品系，该小鼠品系在几乎所有 γ-氨基丁酸 (GABA) 能抑制性神经元中天然表达增强的黄色荧光蛋白 (Venus)，从而能够在整个过程中快速、明确地评估抑制性神经元。 神经系统。我们的结果表明，PNN 在整个听觉中脑和前脑中表达，但其局部分布各不相同。 PNN 在 TRN 中密度最高，在 A1 中密度最低。此外，PNN 优先与 A1 和 TRN 中的抑制性神经元相关，但与小鼠 IC 中的抑制性神经元无关。这些数据表明 PNN 在听觉信息处理中的区域特定作用。
Perineuronal nets (PNNs) are specialized extracellular matrix molecules that are associated with the closing of the critical period, among other functions. In the adult brain, PNNs surround specific types of neurons, however the expression of PNNs in the auditory system of the mouse, particularly at the level of the midbrain and forebrain, has not been fully described. In addition, the association of PNNs with excitatory and inhibitory cell types in these structures remains unknown. Therefore, we sought to investigate the expression of PNNs in the inferior colliculus (IC), thalamic reticular nucleus (TRN) and primary auditory cortex (A1) of the mouse brain by labeling with wisteria floribunda agglutinin (WFA). To aid in the identification of inhibitory neurons in these structures, we employed the vesicular GABA transporter (VGAT)-Venus transgenic mouse strain, which robustly expresses an enhanced yellow-fluorescent protein (Venus) natively in nearly all gamma-amino butyric acid (GABA)-ergic inhibitory neurons, thus enabling a rapid and unambiguous assessment of inhibitory neurons throughout the nervous system. Our results demonstrate that PNNs are expressed throughout the auditory midbrain and forebrain, but vary in their local distribution. PNNs are most dense in the TRN and least dense in A1. Furthermore, PNNs are preferentially associated with inhibitory neurons in A1 and the TRN, but not in the IC of the mouse. These data suggest regionally specific roles for PNNs in auditory information processing.