Depression of Mitogen-Induced Lymphocyte Blastogenesis by Baboon Endogenous Retrovirus-Associated Components 1
Depression of Mitogen-Induced Lymphocyte Blastogenesis by Baboon Endogenous Retrovirus-Associated Components 1
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狒狒内源性逆转录病毒相关成分抑制有丝分裂原诱导的淋巴细胞母细胞生成 1
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
A. Fowler
中科院分区：
文献类型：
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作者：
O. Weislow;Oscar U. Fisher;D. Twardzik;A. Hellman;A. Fowler
狒狒内源性逆转录病毒（BEV）梯度纯化浓缩物的冻融（F/T）提取物抑制植物血凝素（PHA）诱导的人、狒狒和小鼠淋巴细胞的胚性转化。正常胎犬胸腺细胞提取物（用于病毒复制的溶剂）、颗粒状病毒核心、用过的组织培养基和病毒悬浮液缓冲液均未出现抑制。BEV F/T提取物的Sephacryl S-200层析产生两个抑制活性峰;峰A，在具有45，000-道尔顿卵清蛋白标记物的相对高蛋白浓度区域洗脱，是高度不稳定的，但没有细胞毒性。在细胞色素c和DNP丙氨酸标记物之间洗脱的峰B不含可检测的蛋白质，似乎由两种组分组成;一种具有细胞毒性且高度不稳定，另一种无细胞毒性且相对稳定。BEV蛋白质的多价抗血清沉淀BEV F/T提取物的组分和峰A和B，但不能消除免疫抑制。
Abstract Freeze/thaw (F/T) extracts of gradient purified concentrates of baboon endogenous retrovirus (BEV) depress phytohemagglutinin (PHA)-induced blastogenic transformation of human, baboon, and mouse lymphocytes. Extracts of normal fetal dog thymus cells (vehicle for virus replication), pelleted virus cores, spent tissue culture medium, and virus suspension buffers were not depressive. Sephacryl S-200 chromatography of BEV F/T extracts yielded two peaks of suppressive activity; peak A, eluted in the region of relatively high protein concentration with the 45,000-dalton ovalbumin marker, was highly labile, but not cytotoxic. Peak B, eluted between cytochrome c and DNP alanine markers, contained no detectable protein and appeared to consist of two components; one cytotoxic and highly labile and a second, noncytotoxic and relatively stable. A polyvalent antiserum to BEV proteins precipitated components of the BEV F/T extract and peaks A and B, but failed to abrogate immunodepression.