Mapping of a gene that confers short lateral branching (slb) in melon (Cucumis melo L.)
Mapping of a gene that confers short lateral branching (slb) in melon (Cucumis melo L.)
复制标题
DOI：
10.1007/s10681-012-0667-3
复制
发表时间：
2012-03
期刊：
影响因子：
1.9
通讯作者：
N. Fukino;T. Ohara;M. Sugiyama;N. Kubo;Masashi Hirai;Yoshiteru Sakata;S. Matsumoto
中科院分区：
文献类型：
--
作者：
N. Fukino;T. Ohara;M. Sugiyama;N. Kubo;Masashi Hirai;Yoshiteru Sakata;S. Matsumoto
植物结构在许多作物的产量、产品质量和栽培实践中起着重要作用。分支形态是甜瓜植物结构的重要组成部分之一。瓜Chukanbohon now 4 Go （no -4）具有短侧分枝性状，源自杂草甜瓜LB-1。据报道，这种性状是由一个被称为短侧分支（slb）的单隐性或不完全显性主基因控制的。为了寻找该基因的分子标记辅助选择，我们首先利用no4与具有正常分枝的品种“Earl’s Favourite （Harukei-3）”杂交的94株f2植株构建了连锁图谱。然后，我们进行了数量性状位点（QTL）分析，确定了两个短侧支的位点。在连锁组（LG） XI中，no -4等位基因与短侧支相关的一个主要QTL解释了50.9%的表型变异，LOD评分为12.5。我们认为这个QTL对应于slb，因为它的影响很大。在LG III中，Harukei-3等位基因与短侧支相关的另一个小QTL解释了9.9%的表型变异，LOD评分为4.2。利用一个独立群体，我们证明了与该QTL连锁的SSR标记可以用于LG XI （slb）短分支的选择。这是首次报道了调控甜瓜植株结构的基因定位。
Plant architecture plays an important role in the yield, product quality, and cultivation practices of many crops. Branching pattern is one of the most important components in the plant architecture of melon (Cucumis meloL.). ‘Melon Chukanbohon Nou 4 Go’ (Nou-4) has a short-lateral-branching trait derived from a weedy melon, LB-1. This trait is reported to be controlled by a single recessive or incompletely dominant major gene calledshort lateral branching(slb). To find molecular markers for marker-assisted selection of this gene, we first constructed a linkage map using 94 F2plants derived from a cross between Nou-4 and ‘Earl’s Favourite (Harukei-3)’, a cultivar with normal branching. We then conducted quantitative trait locus (QTL) analysis and identified two loci for short lateral branching. A major QTL in linkage group (LG) XI, at which the Nou-4 allele is associated with short lateral branching, explained 50.9 % of the phenotypic variance, with a LOD score of 12.5. We suggest that this QTL corresponds toslbbecause of the magnitude of its effect. Another minor QTL in LG III, at which the Harukei-3 allele is associated with short lateral branching, explained 9.9 % of the phenotypic variance, with a LOD score of 4.2. Using an independent population, we demonstrated that an SSR marker linked to the QTL in LG XI (slb) could be used to select for short lateral branching. This is the first report of mapping a gene regulating the plant architecture of melon.