Genome-wide association analysis reveals genetic loci and candidate genes for meat quality traits in Chinese Laiwu pigs
Genome-wide association analysis reveals genetic loci and candidate genes for meat quality traits in Chinese Laiwu pigs
复制标题
全基因组关联分析揭示了中国莱芜猪肉质性状的遗传位点和候选基因。
DOI：
10.1007/s00335-015-9558-y
复制
发表时间：
2015-04-01
期刊：
影响因子：
2.5
通讯作者：
Huang, Lusheng
中科院分区：
文献类型：
--
作者：
Xiong, Xinwei;Liu, Xianxian;Huang, Lusheng
肉质性状对养猪业具有重要的经济意义，可以通过分子育种方法进行改良。自1994年首次在全基因组范围内对猪进行数量性状基因座（Quantitative Trait Loci，QTLs）定位以来，近20年来，通过家系连锁分析，已经发现了许多与肉质性状相关的QTL。然而，中国纯种或远交群体中肉质性状的遗传变异却知之甚少。为了揭示这一点，我们对中国纯种莱芜猪的10个肉质性状进行了全基因组关联研究。总共鉴定出75个显著性SNP（P < 1.01 x 10（-6））和33个提示性SNP（P < 2.03 x 10（-5））。在SSC 12上，56.22和61.49 M之间的区域B包含一组SNP，其在全基因组显著性水平上与背最长肌（LM）和半膜肌的肉色参数（L*，亮度; a*，红色; B*，黄色）和水分含量相关。SSC 4上的一个区域对LM的含水量和滴水损失也有多效作用。此外，本研究还在4个显著位点上发现了至少5个新的QTL和多个候选基因，包括4连锁MYH基因（MYH 1、MYH 2、MYH 3和MYH 13）、MAL 2、LPAR 1和PRKAG 3。除SSC 12 QTL外，其他QTL可能具有组织特异性。这些结果为中国莱芜猪肉质性状的遗传基础提供了新的见解，本文报道的一些重要SNP可以纳入该品种的选择计划中。
Meat quality traits have economically significant impacts on the pig industry, and can be improved using molecular approaches in pig breeding. Since 1994 when the first genome-wide scan for quantitative trait loci (QTLs) in pig was reported, over the past two decades, numerous QTLs have been identified for meat quality traits by family based linkage analyses. However, little is known about the genetic variants for meat quality traits in Chinese purebred or outbred populations. To unveil it, we performed a genome-wide association study for 10 meat quality traits in Chinese purebred Laiwu pigs. In total, 75 significant SNPs (P < 1.01 x 10(-6)) and 33 suggestive SNPs (P < 2.03 x 10(-5)) were identified. On SSC12, a region between 56.22 and 61.49 Mb harbored a cluster of SNPs that were associated with meat color parameters (L*, lightness; a*, redness; b*, yellowness) and moisture content of longissimus muscle (LM) and semimembranosus muscle at the genome-wide significance level. A region on SSC4 also has pleiotropic effects on moisture content and drip loss of LM. In addition, this study revealed at least five novel QTLs and several candidate genes including 4-linked MYH genes (MYH1, MYH2, MYH3, and MYH13), MAL2, LPAR1, and PRKAG3 at four significant loci. Except for the SSC12 QTL, other QTLs are likely tissue-specific. These results provide new insights into the genetic basis of meat quality traits in Chinese Laiwu pigs and some significant SNPs reported here could be incorporated into the selection programs involving this breed.