Studies of enzymatic oxidation of TMP-fibers and lignin model compounds by a Laccase-Mediator-System using different 14C and 13C techniques
Studies of enzymatic oxidation of TMP-fibers and lignin model compounds by a Laccase-Mediator-System using different 14C and 13C techniques
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DOI：
10.1007/s00226-011-0439-6
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发表时间：
2012-07-01
影响因子：
3.4
通讯作者：
Kharazipour, Alireza
中科院分区：
文献类型：
--
作者：
Euring, Markus;Trojanowski, Jerzy;Kharazipour, Alireza
在这项工作中，研究了TMP纤维(热机械浆)和结构良好的木素模型化合物脱氢聚合物(DHP)在漆酶-介体系统(LMS)作用下，用不同的C-14和C-13方法进行酶催化氧化的结果。这些方法是用DHP(未标记)的核磁共振波谱(C-13-NMR)和测定C-14标记的DHP和TMP纤维的(CO2)-C-14释放。用~(13)C-核磁共振仪定性和定量地分析了LMS处理的DHP模型化合物和TMP纤维的结构变化。(CO2)-C-14的释放数据解释了DHP和TMP纤维的脱甲基化。通过比较只用漆酶处理的DHP纤维和TMP纤维和以灭活的LMS为对照的结果，可以看出LMS的效果。比较C-13-核磁共振法的结果，特别是在酶处理过程中使用介体，发现DHP的结构发生了显著的变化。此外，LMS对TMP纤维也有很大影响。对标记了C-14的DHP和TMP纤维的(CO2)-C-14释放数据的分析表明，在漆酶-介体孵育的第一个小时内，C-14-2原子和(OCH3)-C-14基团的(CO2)-C-14的释放速率都增加了。因此，DHP纤维的(CO2)-C-14释放量高于TMP纤维。
In this work, the results of the enzymatic oxidation of TMP-fibers (thermomechanical pulp) and a well-structured lignin model compound, the dehydropolymer (DHP), were investigated by different C-14 and C-13 methods, caused by a Laccase-Mediator-System (LMS). These methods are the nuclear magnetic resonance spectroscopy (C-13-NMR) with DHP (unmarked) and the determination of the (CO2)-C-14 release of C-14-marked DHP and TMP-fibers. The C-13-NMR measurements were chosen to analyze the structural changes of the LMS-treated DHP model compounds and TMP-fibers qualitatively and quantitatively. The data of (CO2)-C-14 release give an explanation of the demethylation of DHP and TMP-fibers. The effect of the LMS is shown by comparing the results in respect of DHP and TMP-fibers, which were only treated with laccase and of an inactivated LMS as the control. Comparing the results of the C-13-NMR method, in particular the use of the Mediator during the enzymatical treatment, showed significant changes in the structure of the DHP. Also, the TMP-fibers were materially influenced by the LMS. The analysis of the (CO2)-C-14 release data of the C-14-marked DHP and TMP-fibers revealed that the rate of (CO2)-C-14 increases in the C-14-2 atom as well as in the (OCH3)-C-14 group within the first hour of Laccase-Mediator incubation. Therefore, the (CO2)-C-14 release from the DHP was higher than from the TMP-fibers.