Controlled release of neurotrophin-3 from fibrin-based tissue engineering scaffolds enhances neural fiber sprouting following subacute spinal cord injury.
Controlled release of neurotrophin-3 from fibrin-based tissue engineering scaffolds enhances neural fiber sprouting following subacute spinal cord injury.
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DOI：
10.1002/bit.22476
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发表时间：
2009-12-15
影响因子：
3.8
通讯作者：
Sakiyama-Elbert, Shelly E.
中科院分区：
文献类型：
--
作者：
Johnson, Philip J.;Parker, Stanley R.;Sakiyama-Elbert, Shelly E.
本研究探讨纤维蛋白支架控制释放神经营养因子-3 （NT-3）延迟治疗脊髓损伤是否能促进神经纤维发芽。长Evans大鼠T9脊髓背半切损伤。两周后，再次暴露损伤部位，在损伤部位植入单独的纤维蛋白支架、含有不同浓度NT-3（500和1000 ng/mL）的基于肝素的纤维蛋白支架或含有1000 ng/mL NT-3（无递送系统）的纤维蛋白支架。治疗2周后，用神经元、星形胶质细胞和硫酸软骨素蛋白聚糖标记物评估损伤脊髓的形态学差异。与单独使用纤维蛋白相比，在递送系统中添加500 ng/mL NT-3导致神经纤维密度增加。这些结果表明，即使在损伤后延迟治疗2周，纤维蛋白支架中NT-3的控制释放也能促进神经纤维的发芽。
This study investigated whether delayed treatment of spinal cord injury with controlled release of neurotrophin-3 (NT-3) from fibrin scaffolds can stimulate enhanced neural fiber sprouting. Long Evans rats received a T9 dorsal hemisection spinal cord injury. Two weeks later, the injury site was re-exposed, and either a fibrin scaffold alone, a fibrin scaffold containing a heparin-based delivery system with different concentrations of NT-3 (500 and 1000 ng/mL), or a fibrin scaffold containing 1000 ng/mL of NT-3 (no delivery system) was implanted into the injury site. The injured spinal cords were evaluated for morphological differences using markers for neurons, astrocytes, and chondroitin sulfate proteoglycans 2 weeks after treatment. The addition of 500 ng/mL of NT-3 with the delivery system resulted in an increase in neural fiber density compared to fibrin alone. These results demonstrate that the controlled release of NT-3 from fibrin scaffolds can enhance neural fiber sprouting even when treatment is delayed 2 weeks following injury.
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