Inducing RNAi in C. elegans by feeding with dsRNA-expressing E. coli.
Inducing RNAi in C. elegans by feeding with dsRNA-expressing E. coli.
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DOI：
10.1101/pdb.prot072348
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发表时间：
2012-12-01
影响因子：
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通讯作者：
Hannon, Gregory J
中科院分区：
文献类型：
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作者：
Hammell, Christopher M;Hannon, Gregory J
由于摄入介导的RNAi相对容易和高通量的性质，工程大肠杆菌对野生型和突变型秀丽隐杆线虫的饲养已经发展成为最有效和最常用的方法来探测C. elegans基因该方案包括两种通过喂食的RNAi变体：一种是将L1幼虫喂食表达dsRNA的E.在液体培养中的大肠杆菌（通常用于评价RNAi对胚后幼虫发育的影响）和另一种涉及在标准固体培养基培养板上喂养晚期幼虫或成虫阶段的动物。
Owing to the relative ease and high-throughput nature of ingestion-mediated RNAi, the feeding of engineered Escherichia coli to wild-type and mutant Caenorhabditis elegans has developed into the most productive and common method to probe the functions of C. elegans genes. This protocol includes two variations of RNAi by feeding: one in which L1 larvae are fed dsRNA-expressing E. coli in liquid culture (commonly used to score effects of RNAi on postembryonic larval development) and another involving feeding of late larval- or adult-staged animals on standard solid medium culture plates.