The function of the NineTeen Complex (NTC) in regulating spliceosome conformations and fidelity during pre-mRNA splicing.
The function of the NineTeen Complex (NTC) in regulating spliceosome conformations and fidelity during pre-mRNA splicing.
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DOI：
10.1042/bst0381110
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发表时间：
2010-08
影响因子：
3.9
通讯作者：
O'Keefe RT
中科院分区：
文献类型：
--
作者：
Hogg R;McGrail JC;O'Keefe RT
蛋白质的NineTeen复合物（NTC）在前信使RNA剪接期间与剪接体缔合，并且对于内含子去除的两个步骤都是必需的。NTC和其他NTC相关蛋白被募集到剪接体中，在剪接体中它们参与调节两个剪接步骤所需的基本剪接体构象的形成和进展。现在很清楚，NTC是从酵母到人类的活性剪接体的组成部分，并为剪接体snRNP提供必要的支持。在这里，我们讨论了酵母NTC的鉴定和表征，并回顾了最近在酵母中的工作，支持这种复杂的剪接的调节和保真度的重要作用。
The NineTeen Complex (NTC) of proteins associates with the spliceosome during pre-messenger RNA splicing and is essential for both steps of intron removal. The NTC and other NTC associated proteins are recruited to the spliceosome where they participate in regulating the formation and progression of essential spliceosome conformations required for the two steps of splicing. It is now clear that the NTC is an integral component of active spliceosomes from yeast to humans and provides essential support for the spliceosomal snRNPs. Here we discuss the identification and characterisation of the yeast NTC and review recent work in yeast that supports the essential role for this complex in the regulation and fidelity of splicing.