Genome-Wide Investigation of Major Enzyme-Encoding Genes in the Flavonoid Metabolic Pathway in Tartary Buckwheat (Fagopyrum tataricum)
Genome-Wide Investigation of Major Enzyme-Encoding Genes in the Flavonoid Metabolic Pathway in Tartary Buckwheat (Fagopyrum tataricum)
复制标题
苦荞 (Fagopyrum tataricum) 类黄酮代谢途径中主要酶编码基因的基因组广泛研究
DOI：
10.1007/s00239-021-10004-6
复制
发表时间：
2021-03-24
影响因子：
3.9
通讯作者：
Wu, Qi
中科院分区：
文献类型：
--
作者：
Yao, Yingjun;Sun, Lei;Wu, Qi
关键酶在植物生长发育中起着至关重要的作用。然而，苦荞黄酮代谢途径中编码关键酶的基因之间的进化关系尚不清楚。本研究基于已发表的苦荞基因组序列和相关转录组数据，从苦荞基因组中筛选出48个参与类黄酮代谢的关键酶编码基因；研究了这些酶编码基因的染色体定位、基因结构和启动子元件。基因结构分析显示，在48个基因中，5′外显子序列相对保守，说明苦荞中关键酶编码基因的结构多样性较低。通过启动子分析，发现这些关键的酶编码基因含有大量的光响应元件和激素响应元件。此外，部分基因可以结合MYB转录因子，参与类黄酮生物合成的调控。48关键酶编码基因的转录水平在不同组织间差异很大。在这项研究中，我们鉴定了48个参与黄酮类代谢途径的关键酶编码基因，并阐明了这些基因的结构、进化和组织特异性表达模式。这些结果为进一步了解苦荞类黄酮代谢途径关键酶编码基因的功能特征和进化关系奠定了基础。
Key enzymes play a vital role in plant growth and development. However, the evolutionary relationships between genes encoding key enzymes in the metabolic pathway of Tartary buckwheat flavonoids are poorly understood. Based on the published Tartary buckwheat genome sequence and related Tartary buckwheat transcriptome data, 48 key enzyme-encoding genes involved in flavonoid metabolism were screened from the Tartary buckwheat genome in this study; the chromosome localization, gene structure and promoter elements of these enzyme-encoding gene were also investigated. Gene structure analysis revealed relatively conserved 5 ' exon sequences among the 48 genes, indicating that the structural diversity of key enzyme-encoding genes is low in Tartary buckwheat. Through promoter analysis, these key enzyme-encoding genes were found to contain a large number of light-response elements and hormone-response elements. In addition, some genes could bind MYB transcription factors, participating in the regulation of flavonoid biosynthesis. The transcription level of the 48 key enzyme-encoding gene varied greatly among tissues. In this study, we identified 48 key enzyme-encoding genes involved in flavonoid metabolic pathways, and elucidated the structure, evolution and tissue-specific expression patterns of these genes. These results lay a foundation for further understanding the functional characteristics and evolutionary relationships of key enzyme-encoding genes involved in the flavonoid metabolic pathway in Tartary buckwheat.