Changes of tissue creatine concentrations upon oral supplementation of creatine-monohydrate in various animal species
Changes of tissue creatine concentrations upon oral supplementation of creatine-monohydrate in various animal species
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DOI：
10.1016/s0024-3205(01)01268-1
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发表时间：
2001-08-31
期刊：
影响因子：
6.1
通讯作者：
Stöckler-Ipsiroglu, S
中科院分区：
文献类型：
--
作者：
Ipsiroglu, OS;Stromberger, C;Stöckler-Ipsiroglu, S
肌酸是一种营养补充剂，主要应用于作为生能和神经保护底物。不同的补充方案，不同的剂量和持续时间已被应用，但总肌酸（肌酸和磷酸肌酸）的含量在各种感兴趣的器官缺乏系统的研究。我们研究了豚鼠、小鼠和大鼠口服一水肌酸（1.3-2g/kg/d;膳食摄入量的1.4-2.8%）2-8周后，脑、肌肉、心脏、肾脏、肝脏、肺和静脉/门静脉血浆中总肌酸浓度的变化。肌酸和磷酸肌酸含量的分析是通过高效液相色谱形成的。总肌酸测定为肌酸和磷酸肌酸的总和。补充前，脑、骨骼肌和心肌中的总肌酸浓度较高（10-22 μ mol/g湿重），肝、肾和肺中的总肌酸浓度较低（5-8 μ mol/g湿重）。在补充肌酸期间，补充前浓度高的器官中总肌酸的相对增加较低（补充前值的15-55%），补充前浓度低的器官中总肌酸的相对增加较高（补充前值的260-500%）。总肌酸浓度的增加在补充4周后最明显。在肌肉、脑、肾和肺中，
Creatine is a nutritional supplement with major application as ergogenic and neuroprotective substrate. Varying supplementation protocols differing in dosage and duration have been applied but systematic studies of total creatine (creatine and phosphocreatine) content in the various organs of interest are lacking. We investigated changes of total creatine concentrations in brain, muscle, heart, kidney, liver, lung and venous/portal plasma of guinea pigs, mice and rats in response to 2-8 weeks oral creatine-monohydrate supplementation (1.3-2g/kg/d; 1.4-2.8% of dietary intake). Analysis of creatine and phosphocreatine content was pet-formed by high performance liquid chromatography. Total creatine was determined as the sum of creatine and phosphocreatine. Presupplementation total creatine concentrations were high in brain, skeletal and heart muscle (10-22 mu mol/g wet weight), and low in liver, kidney and lung (5-8 mu mol/g wet weight). During creatine supplementation, the relative increase of total creatine was low (15-55% of presupplementation values) in organs with high presupplementation concentrations, and high (260-500% of presupplementation values) in organs with low presupplementation concentrations. The increase of total creatine concentrations was most pronounced after 4 weeks of supplementation. In muscle, brain, kidney and lungs, an additional increase (p