Silica sol-gel/organic hybrid material for protein encapsulated column of capillary electrochromatography.
Silica sol-gel/organic hybrid material for protein encapsulated column of capillary electrochromatography.
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DOI：
10.1016/j.chroma.2004.04.056
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发表时间：
2004-07
期刊：
影响因子：
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通讯作者：
Masaru Kato;Hazuki Saruwatari;K. Sakai-Kato;T. Toyo′oka
中科院分区：
文献类型：
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作者：
Masaru Kato;Hazuki Saruwatari;K. Sakai-Kato;T. Toyo′oka
以明胶或壳聚糖为基质，制备了一种新型溶胶-凝胶/有机杂化复合材料，用于牛血清白蛋白（BSA）毛细管电色谱整体柱。将该复合整体柱用于熔融石英毛细管中BSA的吸附。明胶和壳聚糖的烷氧基硅烷的加入使色氨酸的对映体分离。非常少量的这些聚合物对于对映体分离是有效的。特别是壳聚糖-四甲氧基硅烷整体柱对色氨酸对映体表现出很高的对映选择性，在CEC模式下，α′=t2/t1，t1为快洗脱对映体，t2为次洗脱对映体，其值达到1.15。此外，复合材料表现出更高的稳定性相比，硅胶溶胶-凝胶柱。这些结果表明，溶胶-凝胶/有机混合复合材料可用作CEC BSA封装柱的整体基质。
A new-type of sol–gel/organic hybrid composite material using gelatin or chitosan with tetramethoxysilane was developed for the bovine serum albumin (BSA)-encapsulated monolithic column for capillary electrochromatography (CEC). The composite monolith was used to immobilize BSA in a fused-silica capillary. The addition of gelatin and chitosan to the alkoxysilane enabled the enantioseparation of Trp. A very small amount of these polymers were effective for the enantioseparation. Especially, the monolithic column prepared from chitosan with tetramethoxysilane showed a high enantioselectivity for Trp enantiomers and the value (α′=t2/t1, t1: fast eluted enantiomer, t2: second eluted enantiomer) reached 1.15 on CEC mode. Furthermore, the composite materials exhibited a higher stability compared to the silica sol–gel column. These results showed that the sol–gel/organic hybrid composite was useful as a monolithic matrix for the BSA-encapsulated column for CEC.