Wireless fluorescence capsule for endoscopy using single photon-based detection.
Wireless fluorescence capsule for endoscopy using single photon-based detection.
复制标题
无线荧光胶囊使用基于单光子的检测进行内窥镜检查。
DOI：
10.1038/srep18591
复制
发表时间：
2015-12-18
影响因子：
4.6
通讯作者：
Cumming DR
中科院分区：
文献类型：
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作者：
Al-Rawhani MA;Beeley J;Cumming DR
荧光成像（Fluorescence Imaging，FI）是生物科学和临床医学中的一项重要技术。目前用于体内或体外研究的FI设备昂贵、体积庞大且消耗大量功率，将该技术限制在实验室和医院检查室。在这里，我们提出了一种低功耗的无线荧光内窥镜胶囊，这将为未来的FI系统和应用铺平道路。与现有技术相比，具有增强的灵敏度，我们已经证明该胶囊可以成功地用于通过组织的荧光团标记来成像组织自发荧光和靶向荧光。该胶囊采用了最先进的互补金属氧化物半导体单光子雪崩探测器成像阵列，集成光学隔离，无线技术和低功耗设计。使用时，胶囊仅消耗30.9 mW，并部署非常低水平的468 nm照明。该设备有可能取代高功耗的侵入式光纤内窥镜，并将临床检查范围扩展到十二指肠以下。为了证明我们的胶囊的性能，我们成像荧光幻影纳入主要组织荧光团（黄素）和吸收剂（血红蛋白）。我们还通过在哺乳动物组织上成像20 μM异硫氰酸荧光素（FITC）标记溶液证明了标记物鉴定的实用性。
Fluorescence Imaging (FI) is a powerful technique in biological science and clinical medicine. Current FI devices that are used either for in-vivo or in-vitro studies are expensive, bulky and consume substantial power, confining the technique to laboratories and hospital examination rooms. Here we present a miniaturised wireless fluorescence endoscope capsule with low power consumption that will pave the way for future FI systems and applications. With enhanced sensitivity compared to existing technology we have demonstrated that the capsule can be successfully used to image tissue autofluorescence and targeted fluorescence via fluorophore labelling of tissues. The capsule incorporates a state-of-the-art complementary metal oxide semiconductor single photon avalanche detector imaging array, miniaturised optical isolation, wireless technology and low power design. When in use the capsule consumes only 30.9 mW, and deploys very low-level 468 nm illumination. The device has the potential to replace highly power-hungry intrusive optical fibre based endoscopes and to extend the range of clinical examination below the duodenum. To demonstrate the performance of our capsule, we imaged fluorescence phantoms incorporating principal tissue fluorophores (flavins) and absorbers (haemoglobin). We also demonstrated the utility of marker identification by imaging a 20 μM fluorescein isothiocyanate (FITC) labelling solution on mammalian tissue.