Generation of a C57BL/6 MYC-Driven Mouse Model and Cell Line of Prostate Cancer.
Generation of a C57BL/6 MYC-Driven Mouse Model and Cell Line of Prostate Cancer.
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DOI：
10.1002/pros.23206
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发表时间：
2016-09
期刊：
影响因子：
--
通讯作者：
Pili R
中科院分区：
文献类型：
--
作者：
Ellis L;Ku S;Li Q;Azabdaftari G;Seliski J;Olson B;Netherby CS;Tang DG;Abrams SI;Goodrich DW;Pili R
转基因小鼠模型是反映人类前列腺肿瘤发生及前列腺癌与微环境相互作用的良好工具。使用GEMMs和衍生细胞系是研究前列腺癌发生和发展与相关肿瘤微环境的有力工具。值得注意的是，这些模型提供了快速临床前治疗研究的能力，包括前列腺癌治疗的免疫疗法。将FVB Hi-MYC小鼠与C57BL/6N小鼠回交，建立了C57BL/6基因背景下的Hi-MYC转基因小鼠模型（B6MYC）。此外，利用条件重编程方法，生成了一种新的C57BL/6 MYC驱动的前列腺腺癌细胞系。我们的研究结果表明，与FVB相比，B6MYC的疾病进展明显延迟。目前的数据也表明浸润性免疫细胞存在于癌前病变，前列腺上皮内瘤变（PIN）。此外，对这种免疫浸润的免疫表型分析表明，骨髓源性抑制细胞（MDSC）占主导地位。此外，我们成功地生成了B6MYC-CaP细胞系，并确定该新的PCa细胞系表达管腔上皮谱系的标记物。这个新的前列腺癌模型为理解MYC驱动的前列腺癌与微环境之间的串扰提供了一个新的平台。重要的是，这些模型将是一个理想的工具，以支持免疫疗法的临床发展，以及其他新的治疗策略的前列腺癌治疗。中华医学杂志（英文版），2016。
Transgenic mouse modeling is a favorable tool to reflect human prostate tumorigenesis and interactions between prostate cancer and the microenvironment. The use of GEMMs and derived cell lines represent powerful tools to study prostate cancer initiation and progression with an associated tumor microenvironment. Notably, such models provide the capacity for rapid preclinical therapy studies including immune therapies for prostate cancer treatment. Backcrossing FVB Hi-MYC mice with C57BL/6N mice, we established a Hi-MYC transgenic mouse model on a C57BL/6 background (B6MYC). In addition, using a conditional reprogramming method, a novel C57BL/6 MYC driven prostate adenocarcinoma cell line was generated. Our results demonstrate that disease progression is significantly delayed in B6MYC when compared to their FVB counterparts. Current data also indicates infiltrating immune cells are present in pre-cancer lesions, prostate intraepithelial neoplasia (PIN). Further, immunophenotyping of this immune infiltrate demonstrates the predominant population as myeloid-derived suppressor cells (MDSC). Also, we successfully generated a B6MYC-CaP cell line, and determined that this new PCa cell line express markers of luminal epithelial lineage. This novel model of PCa provides a new platform to understand the cross talk between MYC driven prostate cancer and the microenvironment. Importantly, these models will be an ideal tool to support the clinical development of immunotherapy as well as other novel therapeutic strategies for prostate cancer treatment. Prostate 76:1192–1202, 2016.
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影响因子：
64.5
作者：
Karthaus WR;Iaquinta PJ;Drost J;Gracanin A;van Boxtel R;Wongvipat J;Dowling CM;Gao D;Begthel H;Sachs N;Vries RGJ;Cuppen E;Chen Y;Sawyers CL;Clevers HC
通讯作者：
Clevers HC
影响因子：
64.5
作者：
Gao D;Vela I;Sboner A;Iaquinta PJ;Karthaus WR;Gopalan A;Dowling C;Wanjala JN;Undvall EA;Arora VK;Wongvipat J;Kossai M;Ramazanoglu S;Barboza LP;Di W;Cao Z;Zhang QF;Sirota I;Ran L;MacDonald TY;Beltran H;Mosquera JM;Touijer KA;Scardino PT;Laudone VP;Curtis KR;Rathkopf DE;Morris MJ;Danila DC;Slovin SF;Solomon SB;Eastham JA;Chi P;Carver B;Rubin MA;Scher HI;Clevers H;Sawyers CL;Chen Y
通讯作者：
Chen Y
影响因子：
4.4
作者：
Stewart, Trina J.;Liewehr, David J.;Abrams, Scott I.
通讯作者：
Abrams, Scott I.
影响因子：
50.3
作者：
Wang, SY;Gao, J;Wu, H
通讯作者：
Wu, H
DOI：
10.1016/0960-0760(95)00001-g
发表时间：
1995-05-01
影响因子：
4.1
作者：
BALEY, PA;YOSHIDA, K;THOMPSON, TC
通讯作者：
THOMPSON, TC