HEMOSIDERIN-LIKE PROPERTIES OF FREE-RADICAL-MODIFIED FERRITIN
HEMOSIDERIN-LIKE PROPERTIES OF FREE-RADICAL-MODIFIED FERRITIN
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DOI：
10.1042/bj2400297
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发表时间：
1986-11-15
影响因子：
4.1
通讯作者：
PETERS, TJ
中科院分区：
文献类型：
--
作者：
OCONNELL, MJ;BAUM, H;PETERS, TJ
共轭希夫碱型荧光测定铁耗尽的样品和氯仿提取的人脾含铁血黄素。铁蛋白与脂质体和抗坏血酸的孵育导致形成具有类似荧光性质的化合物。SDS/聚丙烯酰胺凝胶电泳的蛋白质亚基的分析证实，铁蛋白在与抗坏血酸盐孵育中受损。由于以前的研究表明，完整的铁蛋白是耐蛋白水解降解，这表明，含铁血黄素可能是一个产品的氧化反应，涉及铁蛋白和脂质。
Conjugated-Schiff''s-base-type fluorescence was measured in iron-depleted samples and chloroform extracts of human spleen haemosiderin. Incubation of ferritin with liposomes and ascorbate led to the formation of compounds with similar fluorescence properties. Analysis of protein subunits by SDS/polyacrylamide-gel electrophoresis confirmed that ferritin was damaged in incubations with ascorbate. Since previous studies have shown that intact ferritin is resistant to proteolytic degradation, it is suggested that haemosiderin may be a product of oxidative reactions involving ferritin and lipid.