Immunization with recombinant beta-tubulin from Trypanosoma evansi induced protection against T-evansi, T-equiperdum and T-b. brucei infection in mice
Immunization with recombinant beta-tubulin from Trypanosoma evansi induced protection against T-evansi, T-equiperdum and T-b. brucei infection in mice
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DOI：
10.1111/j.1365-3024.2006.00933.x
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发表时间：
2007-04-01
影响因子：
2.2
通讯作者：
Lun, Z. -R.
中科院分区：
文献类型：
--
作者：
Li, S. -Q.;Fung, M. -C.;Lun, Z. -R.
克隆伊氏锥虫β微管蛋白基因（STIB 806），并在大肠杆菌中表达。预测的T. evansi β-tubulin与T. equiperdum，T. B.布氏杆菌、T. cruzi和T. Danilewskyi，但与T.与剑水蚤的同源性仅为51.6%。重组β-微管蛋白在E.杆菌将其纯化并复性用于免疫学研究。用复性的重组β-微管蛋白免疫小鼠，可保护小鼠免受T. evansi STIB 806、T. equiperdum STIB 818和T. B.布氏STIB 940的保护率分别为83.3%、70%和76.7%。重组β-微管蛋白免疫兔血清对T. Evansi，T. equiperdum和T. B.布氏杆菌体外培养。用重组β-微管蛋白免疫的小鼠和兔血清仅识别T。伊文思β-微管蛋白而不是小鼠β-微管蛋白。本研究结果表明，重组T。伊文思β-微管蛋白是用于开发预防由这三种锥虫物种引起的动物锥虫病的疫苗的潜在候选物。
The beta-tubulin gene of Trypanosoma evansi (STIB 806) was cloned and expressed in Escherichia coli. The predicted amino acid sequence of T. evansi beta-tubulin shows 100%, 99.8%, 99.1%, and 98.6% homology with T. equiperdum, T. b. brucei, T. cruzi and T. danilewskyi, respectively, but is diverse from that of T. cyclops, showing only 51.6% of homology. Recombinant beta-tubulin was expressed as inclusion bodies in E. coli. It was purified and renatured for immunological studies. Mice immunized with the renatured recombinant beta-tubulin were protected from lethal challenge with T. evansi STIB 806, T. equiperdum STIB 818 and T. b. brucei STIB 940, showing 83.3%, 70% and 76.7% protection, respectively. Serum collected from the rabbit immunized with recombinant beta-tubulin inhibited the growth of T. evansi, T. equiperdum and T. b. brucei in vitro. Serum from mice and rabbits immunized with recombinant beta-tubulin recognized only T. evansi beta-tubulin and not mouse beta-tubulin. The results of this study demonstrated that the recombinant T. evansi beta-tubulin is a potential candidate for the development of a vaccine to prevent animal trypanosomiasis caused by these three trypanosome species.