THE COMPLETE NUCLEOTIDE-SEQUENCE OF CELL FUSING AGENT (CFA) - HOMOLOGY BETWEEN THE NONSTRUCTURAL PROTEINS ENCODED BY CFA AND THE NONSTRUCTURAL PROTEINS ENCODED BY ARTHROPOD-BORNE FLAVIVIRUSES
THE COMPLETE NUCLEOTIDE-SEQUENCE OF CELL FUSING AGENT (CFA) - HOMOLOGY BETWEEN THE NONSTRUCTURAL PROTEINS ENCODED BY CFA AND THE NONSTRUCTURAL PROTEINS ENCODED BY ARTHROPOD-BORNE FLAVIVIRUSES
复制标题
DOI：
10.1016/0042-6822(92)90575-a
复制
发表时间：
1992-08-01
期刊：
影响因子：
3.7
通讯作者：
STOLLAR, V
中科院分区：
文献类型：
--
作者：
CAMMISAPARKS, H;CISAR, LA;STOLLAR, V
细胞融合剂（CFA）是一种RNA病毒，最初分离自埃及伊蚊细胞系。虽然我们多年前对该病毒的鉴定表明它与黄病毒相似，但与黄病毒属成员没有可检测到的血清学交叉反应。此外，与已充分研究的黄病毒属成员不同，CFA在测试的几种脊椎动物细胞系中均不复制。我们现在已经确定了CFA基因组的核苷酸序列。将CFA多蛋白的预测氨基酸序列与Genbank中的病毒蛋白序列进行比较，已经清楚地表明CFA现在应该归属于黄病毒科，黄病毒属。CFA蛋白与其他黄病毒蛋白的同源性最高的是NS 5（45%）和NS 3（34%）。结构蛋白的同源性很小。因此，CFA仅与存在序列信息的其他黄病毒有远亲关系;然而，就其疏水性图而言，CFA多蛋白和其他黄病毒的多蛋白非常相似。我们认为CFA是一种昆虫病毒，存在于Ae.埃及红细胞系。因此，CFA似乎是第一个被鉴定为昆虫病毒的黄病毒科黄病毒属成员。
Cell fusing agent (CFA) is an RNA virus originally isolated from a line ofAedes aegyptimosquito cells. Although our characterization of the virus many years ago showed that it resembled the flaviviruses, there was no detectable serological cross-reaction with members of the genus flavivirus. Furthermore, unlike the well-studied members of the genus flavivirus, CFA did not replicate in any of several vertebrate cell lines tested. We have now determined the nucleotide sequence of the CFA genome. Comparison of the predicted amino acid sequence of the CFA polyprotein with viral protein sequences in Genbank, has made it apparent that CFA should now be assigned to the family Flaviviridae, genus flavivirus. The homology between CFA proteins and those of other flaviviruses was highest for NS5 (45%) and NS3 (34%). Little homology was found for the structural proteins. Thus, CFA is only distantly related to the other flaviviruses for which there is sequence information; nevertheless, with respect to their hydrophobicity plots, the CFA polyprotein and the polyproteins of other flaviviruses are remarkably similar. We suggest that CFA is an insect virus, which was present in the embryos from which theAe. aegypticell line was established. Thus, CFA seems to be the first member of the family Flaviviridae, genus flavivirus, to be identified as an insect virus.