Genetic mapping reveals a dominant awn-inhibiting gene related to differentiation of the variety anathera in the wild diploid wheat Aegilops tauschii
Genetic mapping reveals a dominant awn-inhibiting gene related to differentiation of the variety anathera in the wild diploid wheat Aegilops tauschii
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DOI：
10.1007/s10709-017-9998-2
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发表时间：
2018-02
期刊：
影响因子：
1.5
通讯作者：
Ryo Nishijima;T. Ikeda;S. Takumi
中科院分区：
文献类型：
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作者：
Ryo Nishijima;T. Ikeda;S. Takumi
节节山羊草是野生小麦的近亲，是普通小麦的 D 基因组供体。伊蚊的亚种和变种。节节花传统上根据花序结构的差异进行分类。然而，在野生小麦近缘种中，其多样化的遗传信息非常有限。品种 anathera 的外稃上没有芒，但这种诊断特征的遗传基础尚不清楚。在Ae中发现顶部和中部穗状花序的芒长度性状存在很大差异。节节核集合中，向东分散的亚系中间穗的芒长度显着小于其他亚系。阐明控制变种无芒表型的位点。 anathera，我们测量了品种间F2作图群体的芒长度，发现F2个体主要根据穗中部的芒长度分为两个群体，即短芒群体和长芒群体，显着符合3:1的分离比，表明单个位点控制无芒表型。无芒基因座Anathera(Antr)被分配到5D染色体短臂的远端区域。使用每个F2个体的芒长度数据进行的数量性状位点分析表明，只有一个主要位点与Antr位于相同的染色体位置。这些结果表明，单个显性等位基因决定了 anathera 品种的无芒诊断特征。 Antrdominant等位基因是小麦及其近缘种中抑制芒伸长的新基因。
Aegilops tauschii, a wild wheat relative, is the D-genome donor of common wheat. Subspecies and varieties ofAe. tauschiiare traditionally classified based on differences in their inflorescence architecture. However, the genetic information for their diversification has been quite limited in the wild wheat relatives. The variety anathera has no awn on the lemma, but the genetic basis for this diagnostic character is unknown. Wide variations in awn length traits at the top and middle spikes were found in theAe. tauschiicore collection, and the awn length at the middle spike was significantly smaller in the eastward-dispersed sublineage than in those in other sublineages. To clarify loci controlling the awnless phenotype of var. anathera, we measured awn length of an intervariety F2mapping population, and found that the F2individuals could be divided into two groups mainly based on the awn length at the middle of spike, namely short and long awn groups, significantly fitting a 3:1 segregation ratio, which indicated that a single locus controls the awnless phenotype. The awnless locus,Anathera(Antr), was assigned to the distal region of the short arm of chromosome 5D. Quantitative trait locus analysis using the awn length data of each F2individual showed that only one major locus was at the same chromosomal position asAntr. These results suggest that a single dominant allele determines the awnless diagnostic character in the variety anathera. TheAntrdominant allele is a novel gene inhibiting awn elongation in wheat and its relatives.