TGF-beta1 resistance is not associated with alterations in TGF-beta type II receptors in immortalized human lung epithelial cells.
TGF-beta1 resistance is not associated with alterations in TGF-beta type II receptors in immortalized human lung epithelial cells.
复制标题
TGF-β1 耐药性与永生化人肺上皮细胞中 TGF-β II 型受体的改变无关。
DOI：
10.1016/s0304-3835(97)04596-5
复制
发表时间：
1997
期刊：
影响因子：
9.7
通讯作者：
Gerwin,BI
中科院分区：
文献类型：
--
作者：
Hamburger,AW;Smith,T;Elliget,K;Hagiwara,K;Gerwin,BI
A frequent cause of TGF-β resistance is decreased expression or mutation of TGF-β receptor Type II (TGFβRII) protein. We previously isolated two isogeneic subclones of the human bronchial epithelial cell line BEAS-2B that are respectively resistant (R.1) or sensitive (S.6) to the growth inhibitory effects of TGF-β1. In this study, we examined TGFβIIR expression, ability to bind TGF-β1, and cDNA sequence in the resistant cell line. Immunofluorescent analyses with antibody to TGFβRII indicated that this protein was expressed at the surface of R.1 cells. Affinity binding studies showed that TGF-β1 bound equally well to the resistant (R.1) and sensitive (S.6) cell lines. PCR cloning and sequencing of TGFβRII cDNA revealed no changes from wild type in the resistant cell line. We conclude that alterations in TGF-β Type II receptor are not responsible for TGF-β resistance in this cell line.
