De novo transcriptome of the muga silkworm, Antheraea assamensis (Helfer)
De novo transcriptome of the muga silkworm, Antheraea assamensis (Helfer)
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DOI：
10.1016/j.gene.2017.02.021
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发表时间：
2017-05-05
期刊：
影响因子：
3.5
通讯作者：
Bora, Utpal
中科院分区：
文献类型：
--
作者：
Chetia, Hasnahana;Kabiraj, Debajyoti;Bora, Utpal
天蚕是印度东北部阿萨姆邦及其邻近丘陵地区特有的一种半驯化家蚕。它是唯一一家生产一种独特的、具有重要商业价值的金色丝绸的生产商，这种丝绸被称为“木加丝绸”。在本研究中，我们首次报道了用楠树叶培育的Asamens的新转录组。通过对来自多个组织的cDNA文库进行高通量测序而产生的短读数，即。通过从头组装管道将家蚕5龄的消化道、丝腺和残体组装成转录本，然后进行功能注释和分类。总共从2.31亿份原始读物中产生了1，21,433份抄本，其中-74%(89,583份)被分配了功能注释或被归类为Pfam/COG/KEGG类别。对差异表达的转录本进行鉴定和比较序列分析，发现了与蚕丝合成相关的候选基因。丝腺因子-1和3，丝胶样转录本等，具有保守的叉头、同源和POU结构域。还鉴定了几个可能具有潜在的抗细菌、抗真菌或抗寄生虫活性的候选抗菌肽。T/A和AT/TA被预测为转录组中含量最丰富的单核苷酸和双核苷酸简单序列重复标记。通过实时定量聚合酶链式反应(QPCR)扩增8个转录本进行转录组验证。这项研究产生的资源将扩大现有的关于Asamens的基因组数据的外围，促进未来对其未知方面的深入研究。(C)2017爱思唯尔B.V.保留所有权利。
Antheraea assamensis (Lepidoptera: Saturniidae), is a semi-domesticated silkworm known to be endemic to Assam and the adjoining hilly areas of Northeast India. It is the only producer of a unique, commercially important variety of golden silk called "muga silk". Herein, we report the de novo transcriptome of A. assamensis reared on Machilus bombycina leaves for the first time. Short reads generated by high throughput sequencing of cDNA libraries from multiple tissues, viz. alimentary canal, silk gland and residual body of the 5th instar of muga silkworm were assembled into transcripts via a de novo assembly pipeline followed by functional annotation and classification. A total of 1,21,433 transcripts were generated from-231 million raw reads of which-74% (89,583) were either allocated a functional annotation or categorized under Pfam/COG/KEGG categories. Identification of differentially expressed transcripts and their comparative sequence analysis revealed candidate genes related to silk synthesis, viz. silk gland factor-1 and 3, sericin-like transcript, etc. with conserved forkhead, homeo- and POU domains. Several candidate anti-microbial peptides which may have potential anti-bacterial, anti-fungal or anti-parasitic activity in A. assamensis were also identified. T/A and AT/TA were predicted to be the most abundant mono- and di-nucleotide simple sequence repeat markers in the transcriptome. Transcriptome validation was carried out by quantitative real-time PCR (qPCR) amplification of eight transcripts. The resources generated by this study will expand the periphery of existing genomic data on A. assamensis facilitating future in-depth studies on its unknown aspects. (C) 2017 Elsevier B.V. All rights reserved.